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Product Details
Synonyms
Protein-arginine deiminase type-4, HL-60 PAD, Peptidylarginine deiminase IV, Protein-arginine deiminase type IV, PADI4, EC:3.5.3.15
Source
Cynomolgus PADI4 Protein, His Tag (PA4-C5143) is expressed from E. coli cells. It contains AA Met 1 - Pro 663 (Accession # A0A2K5USI5-1).
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 76.1 kDa. The protein migrates as 65-80 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).
Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-HPLC.
Formulation
Supplied as 0.2 μm filtered solution in 500 mM NaCl, pH8.0 with glycerol as protectant.
Contact us for customized product form or formulation.
Shipping and Storage
This product is supplied and shipped on dry ice.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
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Performance Data
SDS-PAGE
Cynomolgus PADI4 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-HPLC
The purity of Cynomolgus PADI4 Protein, His Tag (Cat. No. PA4-C5143) was greater than 90% as determined by SEC-HPLC.
Bioactivity
Measured by its ability to catalyze the deamination of benzoyl-arginine ethyl ester. The specific activity is >1500 pmol/min/μg (QC tested).
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