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Product Details
Synonyms
PCSK9, FH3, HCHOLA3, LDLCQ1, NARC1, PC9
Source
Biotinylated Cynomolgus PCSK9 Protein, His,Avitag (PC9-C82E3) is expressed from human 293 cells (HEK293). It contains AA Gln 31 - Gln 692 (Accession # A8T666).
Predicted N-terminus: Gln 31
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 75.1 kDa. The protein migrates as 15 kDa and 65-75 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Biotinylated Cynomolgus PCSK9 Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Biotinylated Cynomolgus PCSK9 Protein, His,Avitag (Cat. No. PC9-C82E3) is more than 90% and the molecular weight of this protein is around 73-95 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Biotinylated Cynomolgus PCSK9 Protein, His,Avitag (Cat. No. PC9-C82E3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human LDL R, Fc Tag (Cat. No. LDR-H5251) with a linear range of 0.06-1 ng/mL (QC tested).
Protocol
Immobilized Cynomolgus LDL R, His Tag (Cat. No. LDR-C5222) at 5 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus PCSK9 Protein, His,Avitag (Cat. No. PC9-C82E3) with a linear range of 0.2-16 ng/mL (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Proprotein convertase subtilisin kexin type 9
- Category：
- Approved Drugs：
8 Details
- Drugs in Clinical Trials：
35 Details
- Highest Development Stage：
Approved
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