Hantaan virus (Orthohantavirus andesense) pre-Gc Protein, His Tag (MALS verified)

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PRN-V52H3-50ug
$465.00
PRN-V52H3-100ug
$785.00
PRN-V52H3-1mg
$2,885.00
PRN-V52H3
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Synonyms

pre-Gc protein

Source

Hantaan virus (Orthohantavirus andesense) pre-Gc Protein, His Tag (PRN-V52H3) is expressed from human 293 cells (HEK293). It contains AA Glu 652 - Asn 1107 (Accession # Q9E006).

Predicted N-terminus: Glu 652

Molecular Characterization

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 52.0 kDa. The protein migrates as 50-60 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method / rFC method.

Purity

>95% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

Formulation

Supplied as 0.2 μm filtered solution in PBS, 0.5 M Arginine, pH7.4 with glycerol as protectant.

Contact us for customized product form or formulation.

Shipping and Storage

This product is supplied and shipped on dry ice.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • The product MUST be stored at -70°C or lower upon receipt;
  • -70°C for 3 months under sterile conditions.

Background

Hantaviruses (genus Orthohantavirus) are enveloped, negative-sense, single-stranded ribonucleic acid (RNA) viruses. The genomes of hantaviruses are composed of tri-segmented single-stranded, negative sense RNA, designated as small (S), medium (M) and large (L) segments. Each segment encodes nucleocapsid protein (NP), glycoprotein precursor (GPC) and RNA-dependent RNA polymerase (RdRp), respectively. GPC is posttranslationally cleaved into Gn and Gc. Glycoproteins Gn and Gc are transmembrane proteins and constitute an envelope with a lipid membrane derived from host cells. Gn and Gc are involved in receptor binding, membrane fusion and induction of protective immunity. The two glycoproteins are presumed to be the major elements involved in the induction of neutralizing antibodies during hantavirus infection.