Product Details
Synonyms
ROR1, NTRKR1
Source
Human / Cynomolgus / Rhesus macaque ROR1 (39-151, Ig-like domain), His Tag (RO1-H5221) is expressed from human 293 cells (HEK293). It contains AA Glu 39 - Gly 151 (Accession # Q01973-1). In the region Glu 39 - Gly 151, the amino acid sequences of Human, Cynomolgus and Rhesus macaque ROR1 are identical.
Predicted N-terminus: Glu 39
Request for sequenceMolecular Characterization

Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 13.7 kDa. The protein migrates as 25-32 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.1 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE

Human / Cynomolgus / Rhesus macaque ROR1 (39-151, Ig-like domain), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS

The purity of Human / Cynomolgus / Rhesus macaque ROR1 (39-151, Ig-like domain), His Tag (Cat. No. RO1-H5221) is more than 95% and the molecular weight of this protein is around 18-28 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA

Immobilized Human / Cynomolgus / Rhesus macaque ROR1 (39-151, Ig-like domain), His Tag (Cat. No. RO1-H5221) at 2 μg/mL (100 μL/well) can bind Anti-ROR1 Antibody, Human IgG1 with a linear range of 0.3-2.4 ng/mL (QC tested).
Protocol
Bioactivity-FACS

2e5 of anti-ROR1 CAR-293 cells were stained with 100 μL of 10 μg/mL of Human / Cynomolgus / Rhesus macaque ROR1 (39-151, Ig-like domain), His Tag (Cat. No. RO1-H5221) and negative control protein respectively, washed and then followed by PE-anti-His Tag and analyzed with FACS (Routinely tested).
Protocol
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Background
Recent Advances
- English Name:
Inactive tyrosine-protein kinase transmembrane receptor ROR1
- Category:
- Approved Drugs:
0 Details
- Drugs in Clinical Trials:
22 Details
- Highest Development Stage:
Phase 3 Clinical
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