Product Details
Features and Advantages
- Native Conformation: Native sequences, tag-free and natural function.
- Reliable Activity: Biological activity calibrated against WHO/NIBSC standards.
- Stringent Quality Control: Protein content, purity, and cell-based bioactivity testing for each batch.
- Lowest Endotoxin level (<0.01 EU/ug).
- Safety Assurance: Sterile filtration through 0.2 μm membrane.
- AOF: Animal origin-free raw materials throughout the production process.
Synonyms
SDF-1, CXCL12, hSDF-1, IRH, hIRH, PBSF, SDF1, SDF1A, SDF1B
Source
Human SDF-1 Protein, Research Grade (SD1-H5116) is expressed from E. coli cells. It contains AA Lys 22 - Lys 89 (Accession # P48061-2).
Predicted N-terminus: Met
Request for sequenceMolecular Characterization

Other Tags and Version Biotin & Other Labeled Version
This protein carries no "tag".
The protein has a calculated MW of 8.1 kDa. The protein migrates as 10 kDa±3 kDa under reducing (R) condition, and 15 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE).
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-HPLC.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
Quality Description
ACRO's Research-grade products are suitable for a wide range of cell culture applications, particularly for research use in academic institutions. These products are sterilized by filtration, followed by lyophilization where applicable. Typical specifications include endotoxin levels of <0.01 EU/μg and purities >95%. Biological activity is calibrated against WHO/NIBSC standards when available.
ACRO's Premium-grade (Pre-GMP) products are characterized by their high quality and enhanced safety profiles, making them ideal for early-stage discovery and manufacturing processes in cell therapy companies. A key advantage is their seamless transition to corresponding GMP-grade versions. Biological activity is calibrated against WHO/NIBSC standards when available. Typical specifications include endotoxin levels of <0.01 EU/μg and purities >95%. In addition, rigorous testing is conducted to ensure the absence of mycoplasma, HCD, and HCP, thereby guaranteeing product safety.
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Performance Data
SDS-PAGE

Human SDF-1 Protein, Research Grade on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-HPLC

The purity of Human SDF-1 Protein, Research Grade (Cat. No. SD1-H5116) was greater than 95% as determined by SEC-HPLC.
Bioactivity-ELISA

Immobilized Human SDF-1 Protein, Research Grade (Cat. No. SD1-H5116) at 5 μg/mL (100 μL/well) can bind Human CXCR4, Fc Tag (Cat. No. CX4-H5269) with a linear range of 0.04-2.5 μg/mL (Routinely tested).
Protocol
Bioactivity-SPR

Human CXCR4 Full Length Protein, Flag,His Tag (Cat. No. CX4-H52D3) immobilized on CM5 Chip can bind Human SDF-1 Protein, Research Grade (Cat. No. SD1-H5116) with an affinity constant of 516 nM as determined in a SPR assay (in presence of Buffer B) (Biacore 8K) (QC tested).
Protocol
Customer Reviews Writing Reviews

Background
Recent Advances
- English Name:
C-X-C motif chemokine ligand 12
- Category:
- Approved Drugs:
0 Details
- Drugs in Clinical Trials:
6 Details
- Highest Development Stage:
Phase 2 Clinical
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