Save Product
Product Details
Synonyms
SECTM1, K12
Source
Human SECTM1, His Tag (SE1-H5227) is expressed from human 293 cells (HEK293). It contains AA Gln 29 - Gly 145 (Accession # AAH17716).
Predicted N-terminus: Gln 29
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 13.5 kDa. The protein migrates as 18-22 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
Customers Also Viewed
Performance Data
SDS-PAGE
Human SECTM1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
Bioactivity-ELISA
Immobilized Human CD7 Protein, Llama IgG2b Fc Tag (Cat. No. CD7-H5258) at 5 μg/mL (100 μL/well) can bind Human SECTM1, His Tag (Cat. No. SE1-H5227) with a linear range of 8-63 ng/mL (QC tested).
Protocol
Bioactivity-SPR
Cynomolgus CD7 Protein, His Tag (Cat. No. CD7-C52H7) immobilized on CM5 Chip can bind Human SECTM1, His Tag (Cat. No. SE1-H5227) with an affinity constant of 24.7 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Biotinylated Cynomolgus CD7 Protein, His,Avitag (Cat. No. CD7-C82E8) immobilized on CM5 Chip can bind Human SECTM1, His Tag (Cat. No. SE1-H5227) with an affinity constant of 12.4 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Bioactivity-FACS
2e5 of Jurkat Cells were stained with 100 μL of 10 μg/mL of Human SECTM1 Protein, His Tag (Cat. No. SE1-H5227) and negative control protein respectively, washed and then followed by His Antibody, PE and analyzed with FACS (Routinely tested).
Protocol
Customer Reviews Writing Reviews
Background
Recent Advances
Contact Us
[North America]: +1 800-810-0816
[Switzerland]: +41 800 040 012
[Asia & Pacific]: +86 400-682-2521
Drug Candidate Licensing
