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SI0-H5254-100ug
$360.00
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SI0-H5254-1mg (500ug X 2)
$2,615.00
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SI0-H5254
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  • Performance Data
  • Reviews & Citations
  • Important Statement
  • Resources

  • Synonyms

    SIGLEC10, MGC126774, PRO940, Siglec10, SLG2

  • Source

    Human Siglec-10 Protein, Fc (L117A, L118A) Tag (SI0-H5254) is expressed from human 293 cells (HEK293). It contains AA Met 17 - Thr 546 (Accession # Q96LC7-1).

    Predicted N-terminus: Met 17

    Request for sequence

  • Molecular Characterization

    Siglec-10 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a Fc (L117A, L118A) tag at the C-terminus. The protein has a calculated MW of 84.6 kDa. The protein migrates as 100-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. Mutations (L117A, L118A/EU number: L234A/L235A) in human immunoglobulin G1 (hIgG1) Fc diminish binding to FcγRI leading to a significant reduction in ADCC and CDC in comparison to wild type human IgG1.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.

  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

Background

The siglecs (sialic acid-binding Ig-like lectins) are a distinct subset of the Ig superfamily with adhesion-molecule-like structure. We describe here a novel member of the siglec protein family that shares a similar structure including five Ig-like domains, a transmembrane domain, and a cytoplasmic tail containing two ITIM-signaling motifs. Siglec-10 was identified through database mining of an asthmatic eosinophil EST library. The Siglec-10-VAP-1 interaction seems to mediate lymphocyte adhesion to endothelium and has the potential to modify the inflammatory microenvironment via the enzymatic end products.