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Product Details
Synonyms
SIGLEC10, MGC126774, PRO940, Siglec10, SLG2
Source
Human Siglec-10, His Tag (SI0-H52H9) is expressed from human 293 cells (HEK293). It contains AA Met 17 - Thr 546 (Accession # Q96LC7-1).
Predicted N-terminus: Met 17
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 60.1 kDa. The protein migrates as 65-80 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
The protein is designed as a dimer.
Endotoxin
Less than 0.02 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in 25 mM MES, 150 mM NaCl, Arginine, pH5.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Performance Data
SDS-PAGE
Human Siglec-10, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human Siglec-10, His Tag (Cat. No. SI0-H52H9) is more than 95% and the molecular weight of this protein is around 135-170 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human Siglec-10, His Tag (Cat. No. SI0-H52H9) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-Human siglec-10 Antibody, Human IgG1 with a linear range of 0.08-2 ng/mL (QC tested).
Protocol
Customer Reviews Writing Reviews
V***w2023-03-17
LikesThe timely fashion that this protein was delivered has helped us greatly. Knowing how reliable and effective Acro Biosystems is with their product delivery is refreshing. Having access to a quick vendor has helped push along studies. The user interface and accessibility of Acro’s website makes it easy to find exactly what we need when we need it. Definitely will continue to purchase from Acro.
A***r2022-10-20 LikesWe have been using this Human Siglec-10 Protein, His Tag as a capture for an electrochemiluminescent immunoassay. The performance of the assay was very consistent; the background was low and signal-to-noise ratio was very good, so we were able to get great sensitivity. We’ve ordered from ACRO Biosystems several times for this assay and every time shipments are very consistent and timely, and the reagent is always in stock. They are also very responsive whenever we have product questions or documentation questions regarding certain lots.- C***e2022-09-23 LikesService with Acro and their customer support is as quick as they are helpful. Product was needed as soon as possible for a study and they helped us out in getting the product shipped out and delivered us within 2 days of purchasing. Customer support personal are as friendly as they are insightful. Never have they disappointed when purchasing any of their products.
Background
Recent Advances
Drug Development Progress- English Name：
Sialic acid-binding Ig-like lectin 10
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
3 Details
- Highest Development Stage：
Phase 3 Clinical
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