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Product Details
Synonyms
CD22, SIGLEC2, BL-CAM, SIGLEC-2, Siglec2, SIGLEC2FLJ22814
Source
Biotinylated Human Siglec-2, Fc,Avitag (SI2-H82F8) is expressed from human 293 cells (HEK293). It contains AA Asp 20 - Arg 687 (Accession # P20273-1).
Predicted N-terminus: Asp 20
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 103.8 kDa. The protein migrates as 130-150 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Endotoxin
Less than 0.2 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Biotinylated Human Siglec-2, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Biotinylated Human Siglec-2, Fc,Avitag (Cat. No. SI2-H82F8) is more than 90% and the molecular weight of this protein is around 235-270 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Biotinylated Human Siglec-2, Fc,Avitag (Cat. No. SI2-H82F8) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Monoclonal Anti-Human CD22 Antibody, Human IgG1 with a linear range of 0.2-39 ng/mL (QC tested).
Protocol
Bioactivity-FACS
2e5 of anti-Siglec-2 CAR-293 cells were stained with 100 μL of 1 μg/mL of Biotinylated Human Siglec-2 / CD22 Protein, Fc,Avitag (Cat. No. SI2-H82F8) and negative control protein respectively, washed and then followed by PE-SA and analyzed with FACS (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
CD22
- Category：
- Approved Drugs：
2 Details
- Drugs in Clinical Trials：
73 Details
- Highest Development Stage：
Approved
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