Save Product
Product Details
Synonym
SHPS1, SIRPA, CD172A, BIT, MFR, MYD1, P84, PTPNS1
Source
Human SIRP alpha, His Tag (SIA-H5225) is expressed from human 293 cells (HEK293). It contains AA Glu 31 - Arg 370 (Accession # NP_001035111).
Predicted N-terminus: Glu 31
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 38.5 kDa. The protein migrates as 47-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
Customers Also Viewed
Performance Data
SDS-PAGE
Human SIRP alpha, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Human SIRP alpha, His Tag (Cat. No. SIA-H5225) is more than 95% and the molecular weight of this protein is around 40-55 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Serial dilutions of Anti-Human CD47 Neutralizing Antibody were added into Human SIRP alpha, His Tag (Cat. No. SIA-H5225): Biotinylated Human CD47, Fc,Avitag (Cat. No. CD7-H82F6) binding reactions. The half maximal inhibitory concentration (IC50) is 0.1245 μg/mL (Routinely tested).
Protocol
Bioactivity-BLI
Loaded Human CD47, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. CD7-H52A5) on Protein A Biosensor, can bind Human SIRP alpha, His Tag (Cat. No. SIA-H5225) with an affinity constant of 1.1 μM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).
Protocol
Immobilized Human CD47, Fc Tag (Cat. No. CD7-H5256) on AHC Biosensor, can bind Human SIRP alpha, His Tag (Cat. No. SIA-H5225) with an affinity constant of 0.72 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Bioactivity-FACS
FACS assay shows that Human SIRP alpha, His Tag (Cat. No. SIA-H5225) can bind to Jurkat cell expressing CD47. The concentration of SIRP alpha used is 1 μg/mL (Routinely tested).
Protocol
FACS analysis shows that the binding of Human SIRP alpha, His Tag (Cat. No. SIA-H5225) to Jurkat expressing CD47 was inhibited by increasing concentration of neutralizing Anti-CD47 antibody. The concentration of SIRP alpha used is 1 μg/mL. IC50=0.2257 μg/mL (Routinely tested).
Protocol
Customer Reviews Writing Reviews
Background
Recent Advances
Drug Development Progress- English Name：
Signal-regulatory protein alpha
- Category：
- Approved Drugs：
2 Details
- Drugs in Clinical Trials：
18 Details
- Highest Development Stage：
Approved
Contact Us
[North America]: +1 800-810-0816
[Switzerland]: +41 800 040 012
[Asia & Pacific]: +86 400-682-2521
Drug Candidate Licensing