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SARS-CoV-2 (COVID-19) S protein RBD, Mouse IgG2a Fc Tag

COADS MS
DS

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Cat. No. / Size
Price
Qty
SPD-C5251-200ug
$720.00
SPD-C5251-1mg (500ug X 2)
$2615.00
SPD-C5251-10mg (5mg X 2)
$19035.00
SPD-C5251-100mg
$190345.00
ETA of in-stock products:2 business days
Sub-Total$ 0
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Product Details

  • Synonym

    Spike, S protein RBD, Spike glycoprotein Receptor-binding domain, S glycoprotein RBD, Spike protein RBD

  • Source

    SARS-CoV-2 S protein RBD, Mouse IgG2a Fc Tag (SPD-C5251) is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Phe 541 (Accession # QHD43416.1).

    Predicted N-terminus: Arg 319

    Request for sequence

  • Molecular Characterization

    Spike RBD Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a mouse IgG2a Fc tag at the C-terminus.

    The protein has a calculated MW of 52.0 kDa. The protein migrates as 55-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Supplied as 0.2 μm filtered solution in 10 mM PB, Arginine, pH7.4.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • Storage

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.

  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    Spike RBD SDS-PAGE

    SARS-CoV-2 S protein RBD, Mouse IgG2a Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • SEC-MALS

    Spike RBD SEC-MALS

    The purity of SARS-CoV-2 S protein RBD, Mouse IgG2a Fc Tag (Cat. No. SPD-C5251) is more than 90% and the molecular weight of this protein is around 110-125 kDa verified by SEC-MALS.

    Report

  • Bioactivity-ELISA

    Spike RBD ELISA

    Immobilized SARS-CoV-2 S protein RBD, Mouse IgG2a Fc Tag (Cat. No. SPD-C5251) at 1 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Neutralizing Antibody, Human IgG1 (Cat. No. SAD-S35) with a linear range of 0.2-3 ng/mL (QC tested).

    Protocol

  • Bioactivity-BLI

    Spike RBD BLI

    Loaded SARS-CoV-2 S protein RBD, Fc Tag (Cat. No. SPD-C5251) on Protein A Biosensor, can bind Human ACE2, His Tag (Cat. No. AC2-H52H8) with an affinity constant of 0.255nM as determined in BLI assay (ForteBio Octet Red96e)(Routinely tested).

    Protocol

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Background

It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

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