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SARS-CoV-2 (COVID-19) S protein RBD, His Tag

COADS MS
DS

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Cat. No. / Size
Price
Qty
SPD-C52H2-200ug
$825.00
SPD-C52H2-1mg
$2945.00
SPD-C52H2-10mg (5mg X 2)
$21425.00
SPD-C52H2-100mg
$214240.00
ETA of in-stock products:2 business days
Sub-Total$ 0
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Product Details

  • This product is specifically designed for serological test development.

  • Synonym

    Spike, S protein RBD, Spike glycoprotein Receptor-binding domain, S glycoprotein RBD, Spike protein RBD

  • Source

    SARS-CoV-2 S protein RBD, His Tag (SPD-C52H2) is expressed from human 293 cells (HEK293).

    Request for sequence

  • Molecular Characterization

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 24.7 kDa. The protein migrates as 30-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Supplied as 0.2 μm filtered solution in PB, pH7.4 .

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • Storage

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.

  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    Spike RBD SDS-PAGE

    SARS-CoV-2 S protein RBD, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

  • Bioactivity-ELISA

    Spike RBD ELISA

    Immobilized SARS-CoV-2 S protein RBD, His Tag (Cat. No. SPD-C52H2) at 1 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 RBD Neutralizing Antibody, Human IgG1 (Cat. No. SAD-S35) with a linear range of 0.2-3 ng/mL (QC tested).

    Protocol

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Background

It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

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