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Product Details
Synonym
Spike, S protein RBD, Spike glycoprotein Receptor-binding domain, S glycoprotein RBD, Spike protein RBD
Source
SARS-CoV-2 S protein RBD (N501Y), His Tag (SPD-C52Hn) is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Lys 537 (Accession # QHD43416.1 (N501Y)). The N501Y mutaion was identified in multiple SARS-CoV-2 Variants of Concerns (VOCs), i.e. the Alpha variant (Pango lineage: B.1.1.7; other names: 20I/501Y.V1 or VOC 202012/01), the Beta variant (Pango lineage: B.1.351; other names: 20H/501Y.V2), the Gamma variant (Pango lineage: P.1; other names: 20J/501Y.V3).
Predicted N-terminus: Arg 319
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 26.6 KDa. The protein migrates as 33-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
SARS-CoV-2 S protein RBD (N501Y), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of SARS-CoV-2 S protein RBD (N501Y), His Tag (Cat. No. SPD-C52Hn) is more than 90% and the molecular weight of this protein is around 28-38 kDa verified by SEC-MALS.
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Bioactivity-ELISA
Immobilized Human ACE2, Fc Tag (Cat. No. AC2-H5257) at 1 μg/mL (100 μL/well) can bind SARS-CoV-2 S protein RBD (N501Y), His Tag (Cat. No. SPD-C52Hn) with a linear range of 2-39 ng/mL (QC tested).
Protocol
Immobilized SARS-CoV-2 S protein RBD (N501Y), His Tag (Cat. No. SPD-C52Hn) at 1 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 RBD Potent Neutralizing Antibody, Human IgG1 (Cat. No. SPD-M180) with a linear range of 0.1-3 ng/mL (Routinely tested).
Protocol
Serial dilutions of Monoclonal Anti-SARS-CoV-2 RBD Potent Neutralizing Antibody, Human IgG1 (Cat. No. SPD-M180) were added into SARS-CoV-2 S protein RBD (N501Y), His Tag (Cat. No. SPD-C52Hn) : Biotinylated Human ACE2, His,Avitag (Cat. No. AC2-H82E6) binding reactions. The half maximal inhibitory concentration (IC50) is 1.13169 (Routinely tested).
Protocol
Bioactivity-SPR
Human ACE2, Fc Tag (Cat. No. AC2-H5257) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind SARS-CoV-2 S protein RBD (N501Y), His Tag (Cat. No. SPD-C52Hn) with an affinity constant of 1.24 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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