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SARS-CoV-2 (COVID-19) S2 protein, His Tag

COADS MS
DS

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Cat. No. / Size
Price
Qty
S2N-C52H2-200ug
$620.00
S2N-C52H2-1mg
$2265.00
S2N-C52H2-10mg
$16480.00
S2N-C52H2-100mg
$164800.00
ETA of in-stock products:2 business days
Sub-Total$ 0
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Product Details

  • Synonym

    Spike, S2 protein, Spike glycoprotein Subunit2, S glycoprotein Subunit2, Spike protein S2

  • Source

    SARS-CoV-2 S2 protein, His Tag (S2N-C52H2) is expressed from human 293 cells (HEK293).

    Request for sequence

  • Molecular Characterization

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 60.0 kDa. The protein migrates as 65-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Supplied as 0.2 μm filtered solution in 20 mM PB, 300 mM NaCl, pH7.4 with glycerol as protectant.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • Storage

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.

  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    Spike S2 SDS-PAGE

    SARS-CoV-2 S2 protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

  • Bioactivity-ELISA

    Spike S2 ELISA

    Immobilized SARS-CoV-2 S2 protein, His Tag (Cat. No. S2N-C52H2) at 0.2 μg/mL (100 ug/well) can bind Anti-SARS-CoV-2 Spike S2 protein Antibody, Human IgG4 (AS86) (Cat. No. S2N-S86) with a linear range of 1.5-2.5 ng/mL (QC tested).

    Protocol

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Background

It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

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