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Product Details
Synonyms
Streptavidin, SA
Source
Streptavidin-PE (STN-NP119) is expressed from E. coli cells.
Molecular Characterization
This protein carries no "tag".
The protein has a calculated MW of 13.8 kDa.
Conjugate
PE
Excitation Wavelength: 488 nm / 561 nm
Emission Wavelength: 575 nm
Application
Flow Cytometry
We also carry another Premium Grade Streptavidin Protein-PE (STN-NP117), which is produced under more rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests, and its performance is carefully validated and tested for compatibility for cell culture use or any other applications in the early preclinical stage.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, 1.5% BSA, 0.03% ProClin300, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 24 months in lyophilized state;
- -20°C for 6 months after reconstitution;
- 2-8 °C for 6 months under sterile conditions after reconstitution.
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Performance Data
Bioactivity-FACS
5e5 of Anti-CD19 CAR-293 cells were stained with 100 μL of 20 μg/mL Biotinylated Human CD19 (20-291), Fc,Avitag, premium grade (Cat. No. CD9-H82F6) and negative control protein respectively, washed and then followed with 2.5 μg/mL of Streptavidin-PE (Cat. No. STN-NP119) and analyzed with FACS. PE signal was used to evaluate the binding activity (QC tested).
Protocol
5e5 of Anti-CD19 CAR-293 cells were stained with 100 μL of 20 μg/mL Biotinylated Human CD19 (20-291), Fc,Avitag, premium grade (Cat. No. CD9-H82F6) and negative control protein respectively, washed and then followed with 2.5 μg/mL of Streptavidin Protein-PE (ACROBiosystems & Brand 1 & Brand 2) and analyzed with GraphPad Prism 5. The results showed that the activity of PE-SA of ACROBiosystems is higher than other two company brands (Routinely tested).
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