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Product Details
Synonyms
SDC1, Syndecan-1, CD138, SYND1, SDC
Source
Human Syndecan-1, Fc Tag (SY1-H5253) is expressed from human 293 cells (HEK293). It contains AA Gln 23 - Gly 254 (Accession # P18827-1).
Predicted N-terminus: Gln 23
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 50.3 kDa. The protein migrates as 65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.2 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human Syndecan-1, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
Bioactivity-ELISA
Immobilized Human Syndecan-1, Fc Tag (Cat. No. SY1-H5253) at 1 μg/mL (100 μL/well) can bind Anti-Syndecan-1 antibody, Mouse IgG1 with a linear range of 0.1-3 ng/mL (QC tested).
Protocol
Immobilized Anti-Syndecan-1 antibody, Mouse IgG1 at 1 μg/mL (100 μL/well) can bind Human Syndecan-1, Fc Tag (Cat. No. SY1-H5253) with a linear range of 0.2-2 ng/mL (Routinely tested).
Protocol
Bioactivity-FACS
2e5 of anti-Syndecan-1 CAR-293 cells were stained with 100 μL of 0.3 μg/mL of Human Syndecan-1, Fc Tag (Cat. No. SY1-H5253) and negative control protein respectively, washed and then followed by PE anti-human IgG Fc antibody and analyzed with FACS (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Syndecan-1
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
2 Details
- Highest Development Stage：
Phase 1 Clinical
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