Human Tau-441 / 2N4R Protein, His Tag, low endotoxin

AIDD‑Ready
Related Products: Cell line CHEK-ATP087
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TAU-H51H3-100ug
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TAU-H51H3-1mg (250ug X 4)
$2,045.00
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Synonyms

DDPAC, FTDP-17, MAPT, MSTD, MTBT1, Tau, PHF-tau, TAU

Source

Human Tau-441, His Tag (TAU-H51H3) is expressed from E. coli cells. It contains AA Met 1 - Leu 441 (Accession # P10636-8).

Predicted N-terminus: Met

Molecular Characterization

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 47.9 kDa. The protein migrates as 65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

Endotoxin

Less than 0.01 EU per μg by the LAL method / rFC method.

Purity

>90% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.

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Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Shipping and Storage

This product is shipped at ambient temperature.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • -20°C to -70°C for 12 months in lyophilized state;
  • -70°C for 3 months under sterile conditions after reconstitution.

Background

Tau is a microtubule-associated protein, which encodes by the MAPT gene that located on chromosome 17q21. Tau Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. Hyperphosphorylation of the tau protein (tau inclusions, pTau) can result in the self-assembly of tangles of paired helical filaments and straight filaments, which are involved in the pathogenesis of Alzheimer's disease, frontotemporal dementia, and other tauopathies. Tau-441 is known as "2N4R," "Isoform Tau-F," "Tau-4" or "Tau 441", which consisting of 441 amino acid. Tau-441 is a potential therapeutic target for pathogenesis.