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Product Details
Synonyms
DIF, TNF-alpha, TNFA, TNFSF2, cachexin, cachectin, TNFα
Source
Rabbit TNF-alpha Protein, His Tag (TNA-R52H4) is expressed from human 293 cells (HEK293). It contains AA Leu 80 - Leu 235 (Accession # P04924).
Predicted N-terminus: Leu 80
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 19.1 kDa. The protein migrates as 19 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).
Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-HPLC.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Rabbit TNF-alpha Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-HPLC
The purity of Rabbit TNF-alpha Protein, His Tag (Cat. No. TNA-R52H4) was greater than 90% as determined by SEC-HPLC.
Bioactivity-ELISA
Immobilized Human TNFR1, Fc Tag (Cat. No. TN1-H5251) at 5 μg/mL (100 μL/well) can bind Rabbit TNF-alpha Protein, His Tag (Cat. No. TNA-R52H4) with a linear range of 2-63 ng/mL (QC tested).
Protocol
Immobilized Human TNFR2 / CD120b / TNFRSF1B Protein, Fc Tag (Cat. No. TN2-H5253) at 5 μg/mL (100 μL/well) can bind Rabbit TNF-alpha Protein, His Tag (Cat. No. TNA-R52H4) with a linear range of 2-250 ng/mL (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Tumor necrosis factor α
- Category：
- Approved Drugs：
68 Details
- Drugs in Clinical Trials：
74 Details
- Highest Development Stage：
Approved
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