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Product Details
Synonyms
TSLP
Source
Mouse TSLP, His Tag (TSP-M52H8) is expressed from human 293 cells (HEK293). It contains AA Tyr 20 - Glu 140 (Accession # Q9JIE6-1).
Predicted N-terminus: Tyr 20
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 15.9 kDa. The protein migrates as 23-27 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Performance Data
SDS-PAGE
Mouse TSLP, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Mouse TSLP, His Tag (Cat. No. TSP-M52H8) is more than 90% and the molecular weight of this protein is around 18-28 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Mouse TSLP, His Tag (Cat. No. TSP-M52H8) at 1 μg/mL (100 μL/well) can bind Recombinant Mouse TSLPR Fc Chimera Protein with a linear range of 0.06-2 ng/mL (QC tested).
Protocol
Customer Reviews Writing Reviews
S***n2023-06-10
LikesWe got the TSLP recombinant protein from your company. The plan was to stimulate cell in vitro to see if it can induce a TH2 response. Your company offers the most cost effective option for the TSLP protein and with good customer service and express shipping. So far we do see some level of responses in the cells, we will perform more testing to validate the effects. The quality of the protein seems quite good and worth trying.
Background
Recent Advances
Drug Development Progress- English Name：
Thymic stromal lymphopoietin
- Category：
- Approved Drugs：
1 Details
- Drugs in Clinical Trials：
29 Details
- Highest Development Stage：
Approved
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