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Product Details
Synonyms
N2DL-1, NKG2DL1, RAET1I
Source
Human ULBP1 Protein, His Tag (UL1-H52H3) is expressed from human 293 cells (HEK293). It contains AA Gly 26 - Pro 215 (Accession # Q9BZM6).
Predicted N-terminus: Gly 26
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 24.2 kDa. The protein migrates as 28-35 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human ULBP1 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human ULBP1 Protein, His Tag (Cat. No. UL1-H52H3) is more than 90% and the molecular weight of this protein is around 25-35 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human NKG2D Protein, Fc Tag (Cat. No. NKD-H5265) at 5 μg/mL (100 μL/well) can bind Human ULBP1 Protein, His Tag (Cat. No. UL1-H52H3) with a linear range of 0.001-0.156 μg/mL μg/mL (QC tested).
Protocol
Bioactivity-SPR
Human ULBP1 Protein, His Tag(Cat. No. UL1-H52H3) immobilized on CM5 Chip can bind Human NKG2D Protein, Fc Tag (Cat. No. NKD-H5265) with an affinity constant of 1.31 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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