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Product Details
Synonyms
VP4 (Rotavirus A)
Source
Rotavirus A (strain RVA/Human/United States/Wa/1974/G1P1A[8]) VP4 Protein, His Tag (VP4-R5243) is expressed from human 293 cells (HEK293). It contains AA Gly 26 - Asn 476 (Accession # P11193).
Predicted N-terminus: His
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 52.9 kDa. The protein migrates as 65-85 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
The protein is designed as a dimer.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Rotavirus A VP4 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Rotavirus A VP4 Protein, His Tag (Cat. No. VP4-R5243) is more than 90% and the molecular weight of this protein is around 145-175 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Rotavirus A VP4 Protein, His Tag (Cat. No. VP4-R5243) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Rotavirus A VP4 Antibody, Human IgG1 (4D2) (Cat. No. VP4-M762) with a linear range of 0.1-2 ng/mL (Routinely tested).
Protocol
Immobilized Rotavirus A VP4 Protein, His Tag (Cat. No. VP4-R5243) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Rotavirus A VP4 Antibody, Human IgG1 (4E1) (Cat. No. VP4-M765) with a linear range of 0.03-2 ng/mL (Routinely tested).
Protocol
Bioactivity-SPR
Monoclonal Anti-Rotavirus A VP4 Antibody, Human IgG1 (4D2) (Cat. No. VP4-M762) captured on Protein A Chip can bind Rotavirus A VP4 Protein, His Tag (Cat. No. VP4-R5243) with an affinity constant of 1.07 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Monoclonal Anti-Rotavirus A VP4 Antibody, Human IgG1 (4E1) (Cat. No. VP4-M765) captured on Protein A Chip can bind Rotavirus A VP4 Protein, His Tag (Cat. No. VP4-R5243) with an affinity constant of 1.25 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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