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Your Position: Home > TNFSF proteins: MALS verified authentic trimer

TNFSF proteins: MALS verified authentic trimer

TNFSF proteins: MALS verified authentic trimer
Background

The tumor necrosis factor (TNF) superfamily plays an important role in regulating immune responses. They exist as trimeric structures in the nature state. The trimer-station of tumor necrosis factor receptor superfamily (TNFRSF) can only take place when TNF-α and TNFR1 binds to each other in the extracellular domain of TNFR1, and effectively stimulate the downstream pathways to induce apoptosis.

The valid aggregation state of TNFSF proteins is critical to its bioactivity and function. Only with correct folding conformation can the proteins effectively ensure the smooth progress of the pre-immunization for antibody drug development, screening and identification, in vitro functional verification, quality control, and clinical sample analysis.

ACROBiosystems has developed a series of TNFSF proteins expressed by HEK293. The TNFSF proteins are in authentic trimeric structure verified by SEC-MALS and suitable for immunization, SPR, cell based assay and more. In addition, the AvitagTM Biotin-labeled proteins are available to accelerate your drug development.

Product features

Authentic trimer structure verified by SEC-MALS

OXL-H52Q8
Human OX40 Ligand /TNFSF4 Protein, His Tag, active trimer

Fig.1 The purity of Human OX40 Ligand, His Tag, active trimer (Cat. No. OXL-H52Q8) was more than 95% and around 60-80 kDa verified by SEC-MALS.

CDL-H5266
Human CD27 Ligand / CD70 Protein, Fc Tag, active trimer

Fig.2 The purity of Human CD27 Ligand, Fc Tag, active trimer (MALS verified, Cat. No. CDL-H5266) was more than 85% and around 160-190 kDa verified by SEC-MALS

High bioactivity verified by cell based assay, SPR, and ELISA

CDL-H5269
Human CD40 Ligand / TNFSF5 Protein, Fc Tag, active trimer

Fig.3 FACS analysis shows that Human CD40 Ligand, Fc Tag, active trimer (MALS verified) (Cat. No. CDL-H5269) can bind to 293T-CD40 cells surface CD40. The concentration of Human CD40 Ligand is 0.3 μg/mL.

TNA-H4211
ActiveMax® Human TNF-alpha Protein, Tag Free, active trimer

Fig.4 ActiveMax® Human TNF-alpha, Tag Free, low endotoxin (active trimer) (MALS verified) (Cat. No. TNA-H4211) induces cytotoxicity effect on the WEH1-13VAR cells in the presence of the metabolic inhibitor actinomycin D. The EC50 for this effect is 0.007-0.014 ng/mL.

41L-H52D4
Human 4-1BB Ligand / TNFSF9 Protein, His,Flag Tag, active trimer

Fig.5 Human 4-1BB Ligand (71-254), His,Flag Tag, active trimer (MALS verified) (Cat. No. 41L-H52D4) induce IL-8 secretion in HT1080 human CD137 cell line. The EC50 for this effect is 0.20-0.44 μg/mL.

LIT-H5242
Human LIGHT /TNFSF14 Protein, His Tag, active trimer

Fig.6 Human LIGHT, His Tag, active trimer (MALS verified) (Cat. No. LIT-H5242) induced cytotoxicity in HT-29 cells. The ED50 for this effect is 1.11-3.87 ng/mL.

RAL-H5240
Human TNFSF11 /RANKL /CD254 Protein, His Tag, active trimer

Fig.7 Captured Human RANK, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. RAK-H5251)  on CM5 chip via Anti-Mouse antibodies surface can bind Human TNFSF11, His Tag, active trimer (Cat. No. RAL-H5240) with an affinity constant of 0.309 nM as determined in a SPR assay (Biacore T200).

CDL-H5266
Human CD27 Ligand / CD70 Protein, Fc Tag, active trimer

Fig.8 Immobilized Human CD27, His Tag (Cat. No. CD7-H522b) at 2 μg/mL (100 μL/well) can bind Human CD27 Ligand, Fc Tag, active trimer (MALS verified) (Cat. No. CDL-H5266) with a linear range of 0.2-8 ng/mL.

Product list
MoleculeCat. No.SpeciesProduct Description
4-1BB Ligand41L-H5265HumanHuman 4-1BB Ligand / TNFSF9 (71-254) Protein, Fc Tag (active trimer) (MALS verified)Hot
41L-H52D4HumanHuman 4-1BB Ligand / TNFSF9 (71-254) Protein, His,Flag Tag (active trimer) (MALS verified)
APRILAPL-H52D1HumanHuman APRIL / TNFSF13 Protein, His,Flag Tag, active trimer (MALS verified)Hot
BAFFBAF-H5261HumanHuman BAFF / TNFSF13B / CD257 Protein, Fc Tag, active trimer (MALS verified)
BAF-H52D4HumanHuman BAFF / TNFSF13B / CD257 Protein, His,Flag Tag, active trimer (MALS verified)
BAF-H82F3HumanBiotinylated Human BAFF / TNFSF13B / CD257 Protein, Avitag™,Fc Tag (active trimer) (MALS verified)
BAF-H82Q2HumanBiotinylated Human BAFF / TNFSF13B / CD257 Protein, His,Avitag™ (active trimer) (MALS verified)
CD27 LigandCDL-H5266HumanHuman CD27 Ligand / CD70 Protein, Fc Tag (active trimer) (MALS verified)Hot
CDL-H52DaHumanHuman CD27 Ligand / CD70 Protein, His,Flag Tag (active trimer) (MALS verified)
CDL-H82D7HumanBiotinylated Human CD27 Ligand / CD70 Protein, His,Avitag™,Flag Tag (active trimer) (MALS verified)
CDL-C5244CynomolgusCynomolgus CD27 Ligand / CD70 Protein, His Tag (active trimer) (MALS verified)
CDL-M5245MouseMouse CD27 Ligand / CD70 Protein, His Tag (active trimer) (MALS verified)
CD40 LigandCDL-H5269HumanHuman CD40 Ligand / TNFSF5 Protein, Fc Tag (active trimer) (MALS verified)Hot
CDL-H52DbHumanHuman CD40 Ligand / TNFSF5 Protein, His,Flag Tag (active trimer) (MALS verified)
CDL-M5248MouseMouse CD40 Ligand / TNFSF5 Protein, His Tag (active trimer) (MALS verified)
Fas LigandFAL-H5241HumanHuman Fas Ligand / TNFSF6 Protein, His Tag (active trimer) (MALS verified)
LIGHTLIT-H5242HumanHuman LIGHT / TNFSF14 Protein, His Tag (active trimer) (MALS verified)
LIT-H5269HumanHuman LIGHT / TNFSF14 Protein, Fc Tag (active trimer) (MALS verified)Hot
OX40 LigandOXL-H52Q8HumanHuman OX40 Ligand / TNFSF4 Protein, His Tag (active trimer) (MALS verified)
TNF-alphaTNA-H4211HumanActiveMax® Human TNF-alpha Protein, Tag Free, low endotoxin (active trimer) (MALS verified)
TNA-H5228HumanHuman TNF-alpha Protein, His Tag (active trimer) (MALS verified)
TNA-H8211HumanBiotinylated Human TNF-alpha Protein, epitope tag free, ultra sensitivity (primary amine labeling) (active trimer) (MALS verified)
TNA-H82E3HumanBiotinylated Human TNF-alpha Protein, His,Avitag™ (active trimer) (MALS verified)
TNA-M82E9MouseBiotinylated Mouse TNF-alpha Protein, His,Avitag™ (active trimer) (MALS verified)
TNFSF11RAL-H5240HumanHuman TNFSF11 / RANKL / CD254 Protein, His Tag (active trimer) (MALS verified)
RAL-H82Q5HumanBiotinylated Human TNFSF11 / RANKL / CD254 Protein, His,Avitag™, active trimer (MALS verified)

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