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Your Position: Home > Protein > IL-7 R alpha > IL7-H82F9

Biotinylated Human IL-7 R alpha / CD127 Protein, Fc,Avitag™

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  • Synonym
    IL7Ra,CD127
  • Source
    Biotinylated Human IL-7 R alpha Protein, Fc,Avitag(IL7-H82F9) is expressed from human 293 cells (HEK293). It contains AA Glu 21 - Asp 239 (Accession # P16871-1 ).
  • Molecular Characterization
    IL-7 R alpha Structure

    This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 53.5 kDa. the protein migrates as 67-75 kDa under reducing (R) condition, and >116 kDa under non-reducing (NR) condition (SDS-PAGE).

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
IL-7 R alpha SDS-PAGE

Biotinylated Human IL-7 R alpha Protein, Fc,Avitag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
 IL-7 R alpha ELISA

Immobilized Human IL-7, Fc Tag (Cat. No. IL7-H5253) at 2 μg/mL (100 μL/well) can bind Biotinylated Human IL-7 R alpha Protein, Fc,Avitag (Cat. No. IL7-H82F9) with a linear range of 1-20 ng/mL (QC tested).

  • Background
    Interleukin-7 receptor subunit alpha (IL7Ra) is also known as CD antigen CD127, which belongs to the type I cytokine receptor family and type 4 subfamily. IL7Ra /CD127 contains 1 fibronectin type-III domain. IL7Ra /CD127 is expressed on various cell types, including naive and memory T cells and many others. IL7Ra /CD127 is receptor for interleukin-7 and also acts as a receptor for thymic stromal lymphopoietin (TSLP). The IL7 receptor is a heterodimer of IL7R and IL2RG. The TSLP receptor is a heterodimer of CRLF2 and IL7R.
  • Clinical and Translational Updates

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