Select Language
Free Registration

Fc Receptors: Key Reagents for Antibody Drug Half-Life Assessment and In Vitro Functional Validation

Why Fc Receptors Matter in Antibody Drug Development
The Fc domain of antibodies is crucial for therapeutic efficacy, safety, and pharmacokinetics. Optimizing interactions with Fc receptors enables you to:
Enhance Therapeutic Efficacy: Improve ADCC, ADCP, and CDC effector functions.
Extend Half-life: Leverage FcRn recycling mechanisms for improved pharmacokinetics.
Reduce Side Effects: Minimize immune-related adverse events like CRS.
We offer a comprehensive suite of Fc receptor-related products to support every stage of antibody drug development.
Fc Receptors Applications in Antibody Drug Development
Antibody Screening and Validation

The neonatal Fc receptor (FcRn) plays a pivotal role in regulating antibody half-life and pharmacokinetics by protecting IgG antibodies from lysosomal degradation through pH-dependent binding and recycling. Early-stage antibody screening focused on FcRn binding affinity is crucial to predict in vivo half-life and optimize dosing strategies.

Our Functional Validation Tools:

FcRn Protein Reagents

TR-FRET FcRn Binding Kits

FcRn Stable Cell Lines

Antibody Fc Region Affinity Evaluation

Evaluating the affinity between an antibody’s Fc region and Fc receptors is crucial for developing effective antibody drugs and immunotherapies. This assessment helps optimize immune responses, improving therapeutic effects while reducing side effects. Proper Fc affinity also enhances antibody stability, efficacy, and half-life, supporting better drug design and personalized treatments.

Our Functional Validation Tools:

FcγR Protein Reagents

TR-FRET FcγR Binding Kits

FcγR Stable Cell Lines

We offer a comprehensive suite of Fc receptor-related products to support every stage of antibody drug development.

Our Solutions

FcR Proteins
FcR Proteins
SPR/BLI-validated high affinity
SEC-MALS verified structural homogeneity
Avitag™ site-specific biotinylation
Multi-species & tag options
HEK293-expressed
TR-FRET Binding Kits
TR-FRET Binding Kits
0.5–1 hour no-wash protocol (HTS compatible)
Comprehensive Validation: Tested across antibody subtypes and drugs
High batch-to-batch consistency
Accurate detection aligning with SPR binding trends
Overexpression Cell Lines
Overexpression Cell Lines
Stable passage over 20 generations
Legally licensed with global commercial use support
Comprehensive application data to support assay development and validation
IND submission-ready (regulatory compliant)

Antibody Screening and Validation

  • FcRn Proteins
  • FcRn Binding Kit (TR-FRET)
  • FcRn Stable cell lines

FcRn Proteins

Assay Data:

SEC-MALS verified homogeneity
SEC-MALS verified homogeneity

The purity of Canine FCGRT&B2M Heterodimer Protein, His Tag&Tag Free (Cat. No. FCM-C52W8) is more than 90% and the molecular weight of this protein is around 43-60 kDa verified by SEC-MALS.

Affinity validated with Herceptin by SPR
Affinity validated with Herceptin by SPR

Immobilized Biotinylated Human FCGRT&B2M Heterodimer Protein, His,Avitag (Cat. No. FCM-H82W7) on SA Chip can bind Herceptin® with an affinity constant of 0.267 μM as determined in a SPR assay (Biacore 8K) (QC tested).

Protocol
Affinity validated with Herceptin by BLI
Affinity validated with Herceptin by BLI

Loaded Cynomolgus / Rhesus macaque FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (Cat. No. FCM-C5284 ) on SA Biosensor via Biotin his antibody, can bind Herceptin with an affinity constant of 0.13 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

Antibody Fc Region Affinity Evaluation

  • Fcγ Receptor Proteins
  • Fcγ Receptor Binding Kits (TR-FRET)
  • FcγR Stable Cell Line

Fcγ Receptor Proteins

We offer a range of Fc receptor proteins and expertise to support antibody function testing and optimization.

Assay Data:

High homogeneity of CD32a was verified by SEC-MALS
High homogeneity of CD32a was verified by SEC-MALS

The purity of Human CD32a (R167), His Tag (Cat. No. CDA-H5221) is more than 90% and the molecular weight of this protein is around 25-35 kDa verified by SEC-MALS.

The affinity between CD64 and Herceptin® was verified by SPR
The affinity between CD64 and Herceptin® was verified by SPR

Human CD64, His Tag (Cat. No. FCA-H52H1) captured on CM5 chip via anti-His antibody can bind Herceptin® with an affinity constant of 4.92 nM as determined in a SPR assay (Biacore 8K) (QC tested).

Protocol
The affinity between CD16a (V176) and Herceptin® was verified by BLI
The affinity between CD16a (V176) and Herceptin® was verified by BLI

Loaded Human CD16a (V176), His Tag (Cat. No. CD8-H52H4) on HIS1K Biosensor, can bind Herceptin with an affinity constant of 0.447 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol
High batch-to-batch consistency
High batch-to-batch consistency

Batch consistency of Human Fc gamma RIIB / CD32b (Cat. No. CDB-H5228). The similarity of Human Fc gamma RIIB / CD32b (Cat. No. CDB-H5228) for different batches is more than 90%.

Product list

FcRn
FcγRIII(CD16)
FcγRII(CD32)
FcγRI (CD64)
FcγRIV(CD16-2)
Others

Resource

  • Background
  • Our Solutions
  • Antibody Screening and Validation
  • Antibody Fc Region Affinity Evaluation
  • Product list
  • Resource