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SARS-CoV-2 Proteins and Inhibitor Screening Kit

SARS-CoV-2 Proteins and Inhibitor Screening Kit
Background

Six strains of coronaviruses (CoVs) that are able to infect humans have been identified until last year. HCoV-OC43, HCoV-229E, HCoV-NL63, and HCoVHKU1 are not highly pathogenic and only cause mild respiratory diseases. SARS-CoV and MERS-CoV have caused two large-scale pandemics. Before efficient antiviral drugs or vaccines are developed for SARS-CoV or MERS-CoV, another outbreak of pneumonia caused by a new coronavirus (SARS-CoV-2) has emerged in Wuhan since December 2019.

Coronaviruses are a group of enveloped positive-stranded RNA viruses that consist of four structural proteins including spike (S) glycoprotein, envelope (E) protein, membrane (M) protein, and nucleocapsid (N) protein. Spike glycoprotein is the most important surface protein of coronavirus, which can mediate the entrance to human respiratory epithelial cells by interacting with cell surface receptor angiotensin-converting enzyme 2 (ACE2).

Trimer Structure S Protein Verified by SEC-MALS

An article published in Science determined a 3.5-angstrom-resolution cryo-electron microscopy structure of the SARS-CoV-2 S trimer in the prefusion conformation. To facilitate medical countermeasure development, ACROBiosystems has developed the  S protein with a trimer structure. The molecular weight and polymer form of the protein was verified by SEC-MALS technology. We also offer high quality SARS-CoV-2 S1, S RBD, N, human ACE2 and other proteins. The bioactivity was verified by SPR and BLI.

Inhibitor Screening Kit

There is no effective treatment for COVID-19 in the market. It is urgent to develop the SARS-CoV-2 inhibitors, for example vaccines, therapeutic antibodies and small molecular compounds against COVID-19. ACRO has developed the SARS-CoV-2 inhibitor screening kit based on the biotinylated ACE2 protein and SARS-CoV-2 S protein RBD. This inhibitor screening ELISA pair is designed to facilitate the identification and characterization of SARS-CoV-2 inhibitors.

Besides the high-quality proteins and kit, ACRO also offers the following benefits:
  1. SPR/ BLI/ ELISA protocols are offered for free.

  2. Buy a large size of ACE2, S, S1, S RBD, N proteins and get a small size one for free. (One time for each end user only till Apr.30).

Data

Purity of SARS-CoV-2 (COVID-19)  S protein is higher than 85%, and the molecular weight is 640-680 kDa as verified by SEC-MALS.

Fig.1 The purity of SARS-CoV-2  S protein (R683A, R685A), His Tag, active trimer (Cat. No. SPN-C52H8) was more than 85% and the molecular weight is around 640-680 kDa as verified by SEC-MALS.

The affinity constant of human ACE2 protein binding to SARS-CoV-2 (COVID-19) S protein  trimer protein was 35.3 nM as verified by SPR.

Fig.2 Human ACE2, Fc Tag (Cat. No. AC2-H5257) Captured on CM5 chip via anti-human IgG Fc antibodies surface can bind SARS-CoV-2  S protein (R683A, R685A), His Tag, active trimer (Cat. No. SPN-C52H8) with an affinity constant of 35.3 nM as determined in a SPR assay (Biacore 8K).

The linear range of biotinylated human ACE2 protein binding to SARS-CoV-2 (COVID-19) S protein  trimer protein was 4-63 ng/mL as verified by ELISA.

Fig.3 Immobilized SARS-CoV-2  S protein (R683A, R685A), His Tag, active trimer (Cat. No. SPN-C52H8) at 2 μg/mL (100 μL/well) can bind Biotinylated Human ACE2, His,Avitag (Cat. No. AC2-H82E6) with a linear range of 4-63 ng/mL.

Product List

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MoleculeCat. No.SpeciesHostProduct DescriptionOrder/Preorder
S protein RBDEP-105SARS-CoV-2
SARS-CoV-2 Inhibitor Screening Kit

Order

S1 proteinTAS-K001SARS-CoV-2
SARS-CoV-2 antibody titer assay kit (Spike protein S1)

Preorder

Expected launch data: Apr. 27

S protein RBDTAS-K002SARS-CoV-2
SARS-CoV-2 antibody titer assay kit (Spike protein RBD)

Preorder

Expected launch data: Apr. 27

S1 proteinMBS-K001SARS-CoV-2
SARS-CoV-2 Spike S1 protein-coupled magnetic beads

Preorder

Expected launch data: Apr. 27

S protein RBDMBS-K002SARS-CoV-2
SARS-CoV-2 Spike protein RBD-coupled magnetic beads

Preorder

Expected launch data: Apr. 27

ACE2AC2-H82E6HumanHEK293Biotinylated Human ACE2 / ACEH Protein, His,Avitag™ (MALS verified)

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AC2-H82F9HumanHEK293Biotinylated Human ACE2 / ACEH Protein, Fc,Avitag™

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AC2-H5257HumanHEK293Human ACE2 / ACEH Protein, Fc Tag (MALS verified)

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AC2-C52H7CynomolgusHEK293Cynomolgus ACE2 / ACEH Protein, His Tag

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AC2-H52H8HumanHEK293Human ACE2 / ACEH Protein, His Tag

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S proteinSPN-C52H4SARS-CoV-2HEK293SARS-CoV-2 (COVID-19)  S protein (R683A, R685A), His Tag

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SPN-C52H8SARS-CoV-2HEK293SARS-CoV-2 (COVID-19)  S protein (R683A, R685A) , His Tag, active trimer (MALS verified)

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S1 proteinS1N-C52H3SARS-CoV-2HEK293SARS-CoV-2 (COVID-19) S1 protein, His Tag

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S1N-C82E8SARS-CoV-2HEK293Biotinylated SARS-CoV-2 (COVID-19) S1 protein, His,Avitag™ (MALS verified)

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S1N-S52H5SARSHEK293SARS S1 protein, His Tag (MALS verified)

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S1N-C52H4SARS-CoV-2HEK293SARS-CoV-2 (COVID-19) S1 protein, His Tag (MALS verified)

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S1N-C5255SARS-CoV-2HEK293SARS-CoV-2 (COVID-19) S1 protein, Fc Tag

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S2 proteinS2N-C52H5SARS-CoV-2HEK293SARS-CoV-2 (COVID-19) S2 protein, His Tag

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S protein RBDSPD-C82E9SARS-CoV-2HEK293Biotinylated SARS-CoV-2 (COVID-19) S protein RBD, His,Avitag™ (MALS verified)

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SPD-C5255SARS-CoV-2HEK293SARS-CoV-2 (COVID-19) S protein RBD, Fc Tag (MALS verified)

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SPD-S52H6SARSHEK293SARS S protein RBD, His Tag (MALS verified)

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SPD-C52H3SARS-CoV-2HEK293SARS-CoV-2 (COVID-19) S protein RBD, His Tag

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Nucleocapsid proteinNUN-C51H9SARS-CoV-2E.coliSARS-CoV-2 (COVID-19) Nucleocapsid protein, His Tag

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NUN-C5227SARS-CoV-2HEK293SARS-CoV-2 (COVID-19) Nucleocapsid protein, His Tag

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ACE2AC2-M5248MouseHEK293Mouse ACE2 / ACEH Protein, His Tag

Preorder

Expected launch date: Apr. 9

AC2-C5245CanisHEK293Canis ACE2 / ACEH Protein, His Tag

Preorder

Expected launch date: Apr. 22

AC2-P5248Paguma larvataHEK293Paguma larvata ACE2 / ACEH Protein, His Tag

Preorder

Expected launch date: Apr. 22

AC2-R5246RatHEK293Rat ACE2 / ACEH Protein, His Tag

Preorder

Expected launch date: Apr. 9

S proteinSPN-S52H5SARSHEK293SARS S protein (R667A), His Tag

Preorder

Expected launch data: Apr. 8

S1 proteinS1N-C5257SARS-CoV-2HEK293SARS-CoV-2 (COVID-19) S1 protein, Mouse IgG2a Fc Tag

Preorder

Expected launch data: Apr. 8

S2 proteinS2N-C55H9SARS-CoV-2InsectCells-HI5SARS-CoV-2 (COVID-19) S2 protein, His Tag (InsectCells-HI5)

Preorder

Expected launch data: Apr. 23

S protein RBDSPD-C5259SARS-CoV-2HEK293SARS-CoV-2 (COVID-19) S protein RBD, Mouse IgG2a Fc Tag

Preorder

Expected launch data: Apr. 9

SPD-S52H4SARS-CoV-2HEK293SARS-CoV-2 (COVID-19) S protein RBD (V367F), His Tag

Preorder

Expected launch data: Apr. 16

SPD-S52H5SARS-CoV-2HEK293SARS-CoV-2 (COVID-19) S protein RBD (N354D), His Tag

Preorder

Expected launch data: Apr. 16

SPD-S52H7SARS-CoV-2HEK293SARS-CoV-2 (COVID-19) S protein RBD (W436R), His Tag

Preorder

Expected launch data: Apr. 16

SPD-S52H8SARS-CoV-2HEK293SARS-CoV-2 (COVID-19) S protein RBD (R408I), His Tag

Preorder

Expected launch data: Apr. 16

SPD-S52H3SARS-CoV-2HEK293SARS-CoV-2 (COVID-19) S protein RBD (N354D, D364Y), His Tag

Preorder

Expected launch data: Apr. 16

3CL-Mpro3CO-C51H5SARS-CoV-2E.coliSARS-CoV-2 (COVID-19) 3CL-Mpro Protein, His Tag

Preorder

Expected launch date: Apr. 14

Envelope proteinENN-C5128SARS-CoV-2E.coliSARS-CoV-2 (COVID-19) Envelope protein, His Tag

Preorder

Expected launch data: Apr. 16

Nucleocapsid proteinNUN-C81Q6SARS-CoV-2E.coliBiotinylated SARS-CoV-2 (COVID-19) Nucleocapsid protein, His,Avitag™

Preorder

Expected launch data: Apr. 9

NUN-M51H5MERSE.coliMERS Nucleocapsid protein, His Tag

Preorder

Expected launch data: Apr. 30

NUN-S5148SARSE.coliSARS Nucleocapsid protein, His Tag

Preorder

Expected launch data: Apr. 30

RNA-dependent RNA polymeraseRNE-C5143SARS-CoV-2E.coliSARS-CoV-2 (COVID-19) RNA-dependent RNA polymerase, His Tag

Preorder

Expected launch data: Apr. 21

Membrane glycoproteinMEN-C5149SARS-CoV-2E.coliSARS-CoV-2 (COVID-19) Membrane glycoprotein Protein, His Tag

Preorder

Expected launch data: Apr. 23

Papain-like ProteasePAE-C5148SARS-CoV-2E.coliSARS-CoV-2 (COVID-19) Papain-like Protease Protein, His Tag

Preorder

Expected launch data: Apr. 21

NSP10NS0-C5147SARS-CoV-2E.coliSARS-CoV-2 (COVID-19) NSP10 Protein, His Tag

Preorder

Expected launch data: Apr. 30

NSP9NS9-C51H4SARS-CoV-2E.coliSARS-CoV-2 (COVID-19) NSP9 Protein, His Tag

Preorder

Expected launch data: Apr. 30

NSP8NS8-C5149SARS-CoV-2E.coliSARS-CoV-2 (COVID-19) NSP8 Protein, His Tag

Preorder

Expected launch data: Apr. 30

NSP2NS2-C51H3SARS-CoV-2E.coliSARS-CoV-2 (COVID-19) NSP2 Protein, His Tag

Preorder

NSP7NS7-C51H6SARS-CoV-2E.coliSARS-CoV-2 (COVID-19) NSP7 Protein, His Tag

Preorder

NSP1NS1-C51H7SARS-CoV-2E.coliSARS-CoV-2 (COVID-19) NSP1 Protein, His Tag

Preorder

NSP15NS5-C5145SARS-CoV-2E.coliSARS-CoV-2 (COVID-19) NSP15 Protein, His Tag

Preorder

Expected launch data: Apr. 29

NSP16 & NSP10NS0-C51W3SARS-CoV-2E.coliSARS-CoV-2 (COVID-19) NSP16&NSP10 Heterodimer Protein, His Tag&Twin Strep Tag

Preorder

Expected launch data: Apr. 29

NSP13NS3-C51H4SARS-CoV-2E.coliSARS-CoV-2 (COVID-19) NSP13 Protein, His Tag

Preorder

Expected launch data: Apr. 29

NSP14NS4-C5144SARS-CoV-2E.coliSARS-CoV-2 (COVID-19) NSP14 Protein, His Tag

Preorder

Expected launch data: Apr. 29

NSP7 & NSP8NS8-C5125SARS-CoV-2E.coliSARS-CoV-2 (COVID-19) NSP7&NSP8 Protein, His Tag

Preorder

Expected launch data: Apr. 29

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