HCoV-NL63 S1 protein, His Tag

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Cat. No. / Size
Price
Qty
SIN-V52H3-100ug
$390.00
SIN-V52H3-1mg (250ug X 4)
$2875.00
ETA of in-stock products:2 business days

Product Details

  • Synonym

    Spike, S1 protein, Spike glycoprotein Subunit1, S glycoprotein Subunit1, Spike protein S1

  • Source

    HCoV-NL63 S1 protein, His Tag (SIN-V52H3) is expressed from human 293 cells (HEK293). It contains AA Cys 19 - Val 717 (Accession # Q6Q1S2-1).

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  • Molecular Characterization

    Spike S1 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 79.5 kDa. The protein migrates as 120-140 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

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  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    Spike S1 SDS-PAGE

    HCoV-NL63 S1 protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

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Background

It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

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