SARS-CoV-2 Related Products

The pandemic caused by SARS-CoV-2 is a public health emergency of international concern. Data from WHO showed that there were more than 7.38 million confirmed cases globally by June 12, 2020. ACROBiosystems has been keeping a close eye on the development of the epidemic situation and accelerating the development of SARS-COV-2 antigen proteins, antibodies, kits and other related products to facilitate the development of serologic tests，therapeutic antibodies and vaccines.

ACROBiosystems has developed more than 60 products related to SARS-CoV-2, including recombinant proteins, antibodies, kits, beads and so on covering the critical targets of SARS-CoV-2. ACROBiosystems has provided core proteins and antibody materials for several in vitro diagnostic companies to support the development of SARS-COV-2 diagnostic kits.

ACROBiosystems has developed a series of Anti-SARS-CoV-2 antibodies, including the matched antibody pairs with high sensitivity and affinity used for antigen detection of diagnostic kit development. Additionally, we also developed the unique neutralizing antibodies isolated from the convalescent patients' serum.

Assay Data

High sensitivity of 12 pg/mL (ELISA assay)

Fig 1 Immobilized Anti- SARS-CoV-2 Nucleocapsid antibody, mouse Mab (Cat. No. NUN-S46) at 4 μg/mL, add increasing concentrations of SARS-CoV-2 Nucleocapsid protein and then add Biotinylated Anti- SARS-CoV-2 Nucleocapsid antibody, mouse Mab (Cat. No. NUN-S47). Detection was performed using HRP-conjugated streptavidin with sensitivity of 12 pg/mL.

Super affinity of 0.01 nM level(BLI assay)

Fig 2 Anti-SARS-CoV-2 Nucleocapsid Antibody, Mouse MAb(Cat. No. NUN-S47) can bind SARS-CoV-2 Nucleocapsid Protein（Cat. No. NUN-C5227) with an affinity constant of 24.7pM determined in BLI assay (ForteBio Octet Red96e)

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SARS-CoV-2 is an enveloped positive-stranded RNA virus that consists of four structural proteins including spike (S) glycoprotein, envelope (E) protein, membrane (M) protein, and nucleocapsid (N) protein. Spike glycoprotein is the most important surface protein of SARS-CoV-2, which can mediate the virus entry into human respiratory epithelial cells by interacting with cell surface receptor angiotensin-converting enzyme 2 (ACE2). S, S1 and S RBD proteins are important targets in the development of SARS-COV-2 therapeutics and vaccines. The quality and application of SARS-COV-2 S, S1, S RBD and human ACE2 and other proteins are the key to the development of antiviral drugs.

ACROBiosystems has developed nearly 50 kinds of high-quality proteins related to SARS-CoV-2 based on HEK293 human cell expression platform. The natural bioactivities and affinities were verified by ELISA/MALS/SPR/BLI, etc.

Assay Data

Fig.3 The purity of SARS-CoV-2 S protein (R683A, R685A), His Tag, active trimer (Cat. No. SPN-C52H8) was more than 80% and around 550-660 kDa verified by SEC-MALS.

Fig.4 Human ACE2, Fc Tag (Cat. No.AC2-H5257) Captured on CM5 chip via anti-human IgG Fc antibodies surface can bind SARS-CoV-2 S protein (R683A, R685A), His Tag, active trimer (Cat. No.SPN-C52H8) with an affinity constant of 35.3 nM as determined in a SPR assay (Biacore 8K).

Protocol

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ACROBiosystems has developed a series of kit products using its high-quality SARS-CoV-2 antigen proteins to improve the efficiency of anti-SARS-CoV-2 drugs and vaccines development. These kit products include SARS-CoV-2 inhibitor screening kits, antibody titer serologic assay kits, antigen detection kit, antigen pre-coated plates and can be used for SARS-CoV-2 inhibitor screening, neutralizing antibody screening, antibody detection, antigen detection.

Assay Data

Fig.5 Inhibition of Human ACE2 [Biotinylated]: SARS-CoV-2 S protein RBD Binding by SARS-CoV-2 inhibitor.

Serial dilutions of SARS-CoV-2 inhibitor (Cat. No. AC2-NA005) (1:2 serial dilutions, from 20 μg/mL to 0.039 μg/mL (180 nM to 0.35 nM)) was added into Biotinylated ACE2: SARS-CoV-2 S protein RBD binding reactions. The assay was performed according to the above described protocol. Background was subtracted from data points prior to log transformation and curve fitting.

* Corresponding buffer set offered.（Cat.No. EBS-001 ELISA Buffer Set-96T）

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ACROBiosystems has developed high-quality S1 protein and RBD protein pre-coupled magnetic beads, which are ready to use and of high stability, high-efficiency target capture and minimal non-specific binding, and can significantly improve the screening efficiency of COVID-19 neutralizing antibody.

Assay Data

Fig.6 Binding of RBD coupled protein to Anti-SARS-CoV-2 S1 antibody：0.1 mg of Beads (1mg/mL, 100 L) was washed for three times and supernatant was removed.

Antibodies of the corresponding concentration of 100 L (10 g/mL~0.039 g/mL) were added. One hour later，fluorescent labeled secondary antibody was added for another one-hour-reaction, the corresponding Binding signal was detected and the Binding curve as obtained.

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>>> Proteins for COVID-19 Serological Test Development

>>> SARS-CoV-2 related kit products

>>> Pre-coupling protein magnetic beads