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Your Position: Home > All Other Proteins > FOLR1 > FO1-HF2H8

FITC-Labeled Human FOLR1 Protein, His Tag (MALS verified)

  • Synonym
    FOLR-1,FBP,FOLR
  • Source
    FITC-Labeled Human FOLR1, His Tag (FO1-HF2H8) is expressed from human 293 cells (HEK293). It contains AA Arg 25 - Met 233 (Accession # P15328-1). It is the FITC labeled form of Human FOLR1, His Tag (FO1-H52H1).
    Predicted N-terminus: Arg 25
  • Molecular Characterization
    Online(Arg 25 - Met 233) P15328-1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 26.5 kDa. The protein migrates as 35-43 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Conjugate

    FITC

    Excitation source: 488 nm spectral line, argon-ion laser

    Excitation Wavelength: 488 nm

    Emission Wavelength: 535 nm

  • Labeling
    The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular seive treatment during purification process.
  • FITC:Protein Ratio
    The FITC to protein molar ratio is 1.5-3.5.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
FITC-Labeled Human FOLR1, His Tag (Cat. No. FO1-HF2H8) SDS-PAGE gel

FITC-Labeled Human FOLR1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
FITC-Labeled Human FOLR1, His Tag (Cat. No. ) MALS images

The purity of FITC-Labeled Human FOLR1, His Tag (Cat. No. FO1-HF2H8) was more than 90% and the molecular weight of this protein is around 40-50 kDa verified by SEC-MALS.

Bioactivity-ELISA
FITC-Labeled Human FOLR1, His TagFITC-Labeled Human FOLR1, His Tag (Cat. No. FO1-HF2H8) ELISA bioactivity

Immobilized Folic acid-BSA conjugate at 5 μg/mL (100 μL/well) can bind FITC-Labeled Human FOLR1, His Tag (Cat. No. FO1-HF2H8) with a linear range of 0.078-1.25 μg/mL (QC tested).

Bioactivity-FACS
FACS assay shows that recombinant Biotinylated FACS

2e5 of Anti-FOLR1 CAR-293 cells were stained with 100 μL of 3 μg/mL of FITC-Labeled Human FOLR1, His Tag (Cat. No. FO1-HF2H8) and negative control protein respectively, FITC signals was used to evaluate the binding activity (QC tested).

  • Background
    Folate Receptor 1 (FOLR1) is also known as Folate receptor alpha, Folate Binding Protein (FBP), FOLR, and is a member of the folate receptor (FOLR) family. Members of this gene family have a high affinity for folic acid and for several reduced folic acid derivatives, and mediate delivery of 5-methyltetrahydrofolate to the interior of cells. Mature FOLR1 is an N-glycosylated protein that is anchored to the cell surface by a GPI linkage. FOLR1 is predominantly expressed on epithelial cells and is dramatically upregulated on many carcinomas. FOLR1 is internalized to the endosomal system where it dissociates from its ligand before recycling to the cell surface. A soluble form of FOLR1 can be proteolytically shed from the cell surface into the serum and breast milk. Defects in FOLR1 are the cause of neurodegeneration due to cerebral folate transport deficiency (NCFTD). NCFTD is an autosomal recessive disorder resulting from brain-specific folate deficiency early in life.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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