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This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 26.0 kDa. The protein migrates as 30-35 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
>98% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human PD-L1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 98% (With Star Ribbon Pre-stained Protein Marker).
The purity of Human PD-L1, His Tag (Cat. No. PD1-H5229) is more than 90% and the molecular weight of this protein is around 30-50 kDa verified by SEC-MALS.
Immobilized Human PD-L1, His Tag (Cat. No. PD1-H5229) at 1 μg/mL (100 μL/well) can bind Anti-Human PD-L1 MAb (Human IgG1) with a linear range of 0.1-3 ng/mL (QC tested).
Immobilized Human PD-L1, His Tag (Cat. No. PD1-H5229) at series of concentration on MonoRabᵀᴹ Anti-His Tag (C-term) Antibody precoated (0.1 μg/well) plate, can bind Human PD-1, Mouse IgG2a Fc Tag (Cat. No. PD1-H5255) at 5 μg/mL (100 μL/well) with a linear range of 10-78 ng/mL (Routinely tested).
Human PD-1, Fc Tag (Cat. No. PD1-H5257) captured on CM5 chip via anti-human IgG Fc antibody, can bind Human PD-L1, His Tag (Cat. No. PD1-H5229) with an affinity constant of 3.6 μM as determined in a SPR assay (Biacore T200) (Routinely tested).
Anti-Human PD-L1 Mab (Human IgG1) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human PD-L1, His Tag (Cat. No. PD1-H5229) with an affinity constant of 0.286 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Loaded Human PD-1, Fc Tag, low endotoxin (Cat. No. PD1-H5257) on ProteinA Biosensor, can bind Human PD-L1, His Tag (Cat. No. PD1-H5229) with an affinity constant of 5.3 μM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).
Loaded Anti-Human PD-L1 MAb (Human IgG1) on AHC Biosensor, can bind Human PD-L1, His Tag (Cat. No. PD1-H5229) with an affinity constant of 1.61 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
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