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Your Position: Home > All Other Proteins > CD19 > Human CD19 (20-278) Protein, With C-Fc Tag

Human CD19 (20-278) Protein, Fc Tag

  • Synonym
    CD19,B4,CVID3,MGC12802
  • Source
    Human CD19 (20-278), Fc Tag (CD9-H5255) is expressed from human 293 cells (HEK293). It contains AA Pro 20 - Pro 278 (Accession # AAH06338).
    Predicted N-terminus: Pro 20
  • Molecular Characterization
    CD19(Pro 20 - Pro 278) AAH06338

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 55.1 kDa. The protein migrates as 62-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human CD19 (20-278), Fc Tag (Cat. No. CD9-H5255) SDS-PAGE gel

Human CD19 (20-278), Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
Human CD19 (20-278), Fc TagHuman CD19 (20-278), Fc Tag (Cat. No. CD9-H5255) ELISA bioactivity

Immobilized FMC63 MAb at 2μg/mL (100 μL/well) can bind Human CD19, Fc Tag (Cat. No. CD9-H5255) with a linear range of 0.15-1.25 μg/mL (QC tested).

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