Monoclonal Anti-Human CD3 Antibody, Mouse IgG1 (SP34-2)

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Cat. No. / Size
Price
Qty
CDE-M531-100ug
$145.00
CDE-M531-1mg
$620.00
ETA of in-stock products:2 business days

Product Details

  • Source

    Monoclonal Anti-Human CD3 Antibody, Mouse IgG1 (SP34-2) is a monoclonal antibody recombinantly expressed from human 293 cells (HEK293), which provides higher batch consistency and long term security of supply.

  • Antibody Type

    Recombinant Monoclonal

  • Clone

    SP34-2

  • Isotype

    Mouse IgG1, Kappa

  • Host Species

    Mouse

  • Reactivity

    Human

  • Immunogen

    Purified Human CD3ε Protein

  • Specificity

    This product is a specific antibody specifically reacts with CD3 epsilon protein.

  • Application

    ELISA (Evaluation of levels of CD3 epsilon protein).

    Flow Cytometry (Evaluation of an epitope within the extracellular domain of CD3 epsilon protein).

  • Form

    Liquid

  • Formulation

    Supplied as 0.2 μm-filtered solution in PBS (pH 7.4) containing 10% glycerol, with trehalose as protectant.

    Please contact us for customized product forms or formulations.

  • Storage

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 12 months under sterile conditions.
  • Notices

    Product Specific Notices: For research use only.

  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • Bioactivity-FACS

     CD3 FACS

    2e5 of Jurkat cells were stained with 100 μL of 10 μg/mL of Monoclonal Anti-Human CD3 Antibody, Mouse IgG1 (SP34-2) (Cat. No. CDE-M531) and isotype control antibody respectively, washed and then followed by PE-anti mouse IgG1 antibody and analyzed with FACS (Routinely tested).

    Protocol
  • Bioactivity-ELISA

     CD3 ELISA

    Immobilized Human CD3 epsilon, His Tag (Cat. No. CDE-H5223) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human CD3 Antibody, Mouse IgG1 (SP34-2) (Cat. No. CDE-M531) with a linear range of 0.4-6 ng/mL (QC tested).

    Protocol

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Background

CD3e molecule, epsilon is also known as CD3E, is a T-cell surface single-pass type I membrane glycoprotein. CD3E contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3E, together with CD3-gamma, CD3-delta and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. CD3E plays an essential role in T-cell development, and defects in CD3E gene cause severe immunodeficiency. CD3E gene has also been linked to a susceptibility to type I diabetes in women. CD3E has been shown to interact with TOP2B, CD3EAP and NCK2.

Recent Advances

 
Drug Development Progress
  • English Name:

    T cell surface glycoprotein CD3

  • Category:

  • Approved Drugs:

    10 Details

  • Drugs in Clinical Trials:

    198 Details

  • Highest Development Stage:

    Approved

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