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PE-Labeled Monoclonal Anti-Human CD69 Antibody, Mouse IgG1 (3C5B7) (0.03% Proclin)

COADS MS
DS

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Cat. No. / Size
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FABm022-01-200tests (50tests X 4)
$200.00
ETA of in-stock products:2 business days
Sub-Total$ 0
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Product Details

  • Source

    PE-Labeled Monoclonal Anti-Human CD69 Antibody, Mouse IgG1 (3C5B7) is a mouse monoclonal antibody recombinantly expressed from Hybridoma.

  • Antibody Type

    Hybridoma Monoclonal

  • Clone

    3C5B7

  • Isotype

    Mouse IgG1, Kappa

  • Host Species

    Mouse

  • Reactivity

    Human

  • Immunogen

    Purified Human CD69 Protein

  • Conjugate

    PE

    Excitation Wavelength: 488 nm / 561 nm

    Emission Wavelength: 575 nm

  • Specificity

    This product is a specific antibody specifically reacts with CD69 protein.

  • Application

    Flow Cytometry (Evaluation of the expression of CD69 on Human cells).

  • Recommended Dilution

    1:20

  • Isotype Control

    The isotype control is sold separately; please refer to Cat. No. DNP-PM1 for product information.

  • Form

    Liquid

  • Formulation

    Supplied as 0.2 μm-filtered solution in PBS (pH 7.4) containing 0.03% ProClin 300 and 0.2% BSA, with trehalose as protectant.

    Please contact us for customized product forms or formulations.

  • Shipping

    Liquid product is shipped at blue ice.

  • Storage

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. Store at 2-8 °C for 12 months.

  • Notices

    Product Specific Notices: For research use only.

  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • Bioactivity-FACS

    CD69 FACS

    Flow cytometric analysis of Jurkat staining with PE-Labeled Monoclonal Anti-Human CD69 Antibody, Mouse IgG1 (3C5B7) (Cat. No. FABm022-01) at 1:20 dilution (5 μL of the antibody stock solution corresponds to labeling of 1e6 cells in a final volume of 100 µL), compared with isotype control antibody. PE signal was used to evaluate the binding activity (QC tested).

    Protocol

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Background

The activation of T lymphocytes involves a wide variety of cellular changes. Some of these changes are biochemical in nature, such as phosphatidylinositol catabolism and the elevationof cellular calcium. Others are genetic, involving the induction of specific gene expression. Among the constellation of genes induced following T cell activation are several cell surface molecules. One of the earliest of these to be expressed is CD69 (previously known as activation inducer molecule or AIM, EA-1, Leu-23,and MLR3). Within one to two h of activation, CD69 can be found on the cell surface, with expression persisting at least three days. Studies with monoclonal antibodies (MAb) to CD69 have shown that it is a costimulatory molecule for T cell proliferation. In addition, CD69 expression can be induced on most, if not all, cells of hematopoietic origin.

Recent Advances

 
Drug Development Progress
  • English Name：

    Early activation antigen CD69

  • Category：

  • Approved Drugs：

    0 Details

  • Drugs in Clinical Trials：

    0 Details

  • Highest Development Stage：

    Preclinical

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