PE-Labeled Monoclonal Anti-G4S linker Antibody, Rabbit IgG (016) (0.03% Proclin)

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Cat. No. / Size
Price
Qty
G4S-PFMY25-25tests
$280.00
G4S-PFMY25-200tests
$1540.00
G4S-PFMY25-1000tests (200tests X 5)
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ETA of in-stock products:2 business days

Product Details

  • Source

    Monoclonal Anti-G4S linker Antibody, Rabbit IgG (016) is a rabbit monoclonal antibody recombinantly expressed from human 293 cells (HEK293), which provides higher batch consistency and long term security of supply. It shows superior performance in flow cytometry assays with higher affinity and better specificity.

  • Antibody Type

    Recombinant Monoclonal

  • Clone

    016

  • Isotype

    Rabbit IgG, Kappa

  • Host Species

    Rabbit

  • Conjugate

    PE

    Excitation Wavelength: 488 nm / 561 nm

    Emission Wavelength: 575 nm

  • Specificity

    It can specifically recognize proteins or ScFv containing (G3S)n or (G4S)n linkers, where n = 2, 3, 4... That is, it can recognize linkers with a minimum length of 2 repeats for both (G3S)n and (G4S)n.

  • Application

    Flow Cytometry (Evaluation of cell surface expressed CARs of varying specificity containing a G4S linker within the scFv of the extracellular domain).

  • Recommended Dilution

    1:50

  • Form

    Lyophilized

  • Formulation

    Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4) containing 0.03% ProClin 300 and 0.2% BSA, with trehalose as protectant.

    Please contact us for customized product forms or formulations.

  • Reconstitution

    Please refer to the Certificate of Analysis (CoA) for specific instructions.

  • Shipping

    Lyophilized product is shipped at ambient temperature.

  • Storage

    For long term storage, the product should be stored in a lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 24 months in lyophilized state;
    2. -70°C for 12 months after reconstitution.
    3. 2-8°C for 12 months after reconstitution.
  • Notices

    Product Specific Notices: For research use only.

  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • Bioactivity-FACS

     G4S linker FACS

    Flow cytometric analysis of Anti-MSLN CAR-293 cells staining with PE-Labeled Monoclonal Anti-G4S linker Antibody, Rabbit IgG (016) (Cat. No. G4S-PFMY25) at 1:50 dilution (2 μL of the antibody stock solution corresponds to labeling of 1e6 cells in a final volume of 100 µL), compared withisotype control antibody. PE signal was used to evaluate the binding activity (QC tested).

    Protocol
  •  G4S linker FACS

    Non-specificity of PE-Labeled Monoclonal Anti-G4S linker Antibody, Rabbit IgG (016) (Cat. No. G4S-PFMY25) binding to CD3+ cellspresent in human PBMC. 5e5 of human PBMCs were simultaneously stained with FITC-labeled anti-CD3 antibody and PE-Labeled Anti-G4S Linker Antibody (016) (2 μL of the antibody stock solution corresponds to labeling of 5e5 cells in a final volume of 100 µL) andwashed and then analyzed with FACS. Both FITC and PE positive signals was used to evaluate the non-specific binding activity to humanCD3+ cells (QC tested).

    Protocol
  • Typical Data

     G4S linker TYPICAL DATA

    Immunohistochemical analysis of paraffin embedded anti-CD19 CAR-T cells labelled with G4S-PFMY25 at 1/400 dilution.
    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with lHC staining protocol.

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Background

The G4S linker is emerged as the most common linking peptides for scFvs. It is composed of the repeated sequence (Gly-Gly-Gly-Gly-Ser) and commonly found as either a 15-mer (G4S)3 or 20-mer(G4S)4 within scFv fragments. The G4S linker has become an integral part of the vast majority of CARs as it links the VH and VL recognition domains of scFvs. The G4S linker is commonly used in CARs targeting solid tumor antigens, such as HER2 and CEA, while the whitlow/218 linker peptide is predominantly used in CAR constructs targeting hematological malignancies such as CD19 CAR products.

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