Product Details
Source
Monoclonal Anti-HA-H3N2(Influenza A/Darwin/6/2021) Antibody, Mouse IgG1 (13A5) is a mouse monoclonal antibody derived from hybridoma cells fused with SP2/0 myeloma cells and murine B lymphocytes.
Antibody Type
Hybridoma Monoclonal
Clone
13A5
Isotype
Mouse IgG1, Kappa
Host Species
Mouse
Specificity
This product is a specific antibody specifically reacts with HA.
Cross Verification
This product can cross in Elisa with
Influenza A [A/Darwin/6/2021 (H3N2)] HA Protein, His Tag (Cat. No. HA2-V52H5).
Influenza A [A/Darwin/9/2021 (H3N2)] HA Protein, His Tag (Cat. No. HA2-V52H6).
Influenza A Virus HA (H3N2) Protein, His Tag (Cat. No. H32-V52H3).
This product No cross-reactivity in ELISA with
Influenza A [Victoria/4897/2022] Hemagglutinin (HA) Protein, His Tag (Cat. No. HA1-V52H8).
Influenza A [Wisconsin/67/2022] Hemagglutinin (HA) Protein, His Tag (Cat. No. HA1-V52H7).
Influenza A [A/Victoria/2570/2019] Hemagglutinin (HA) Protein, His Tag (Cat. No. HA1-V52H6).
Influenza A [Sydney/5/2021 (H1N1)] HA Protein, His Tag (Cat. No. HA1-V52H4).
Influenza A (Vietnam/1194/2004(H5N1)) Hemagglutinin (HA) Protein, His Tag (Cat. No. HA1-V52H9).
Influenza A (Guangdong/18SF020(H5N6)) Hemagglutinin (HA) Protein, His Tag (Cat. No.HA6-V52H3).
Influenza A (turkey/Germany-MV/R2472/2014(H5N8)) HA Protein, His Tag (Cat. No. HA8-V52H3).
Influenza A (A/Shanghai/02/2013(H7N9)) Hemagglutinin (HA) Protein, His Tag (Cat. No. HA9-V52H3).
Influenza A [A/guinea fowl/Hong Kong/WF10/99(H9N2)] Hemagglutinin (HA) Protein, His Tag (Cat. No. HA2-V52H7).
Influenza B [Austria/1359417/2021 (B/Victoria lineage)] Hemagglutinin (HA) Protein, His Tag (Cat. No. HAE-V52H3).
Influenza B [Phuket/3073/2013 (B/Yamagata lineage)] HA Protein, His Tag (Cat. No. HAE-V52H4).
Influenza A [A/Bangkok/1/1979 (H3N2)] HA, His Tag (Cat. No. HA2-V52H3).
Influenza A [A/Wisconsin/588/2019 (H1N1)] HA, His Tag (Cat. No. HA1-V52H3).
Influenza A [A/Hong Kong/483/97 (H5N1)] HA, His Tag (Cat. No. HA1-V5229).Purification
Protein A purified / Protein G purified.
Concentration
Please refer to the Certificate of Analysis (CoA).
Form
Lyophilized
Formulation
Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.
Please contact us for customized product forms or formulations.
Reconstitution
Please refer to the Certificate of Analysis (CoA) for specific instructions.
Shipping
Lyophilized product is shipped at ambient temperature.
Storage
For long term storage, the product should be stored in a lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Notices
Product Specific Notices: For research use only.
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Performance Data
Bioactivity-ELISA

Immobilized Influenza A [A/Darwin/6/2021 (H3N2)] HA Protein, His Tag (Cat. No. HA2-V52H5) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-HA-H3N2(Influenza A/Darwin/6/2021) Antibody, Mouse IgG1 (13A5) (Cat. No. HA2-Y290) with a linear range of 0.1-6 ng/mL (QC tested).
Protocol
Immobilized Influenza A [A/Darwin/9/2021 (H3N2)] HA Protein, His Tag (Cat. No. HA2-V52H6) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-HA-H3N2(Influenza A/Darwin/6/2021) Antibody, Mouse IgG1 (13A5) (Cat. No. HA2-Y290) with a linear range of 0.2-13 ng/mL (Routinely tested).
Protocol
Immobilized Influenza A Virus HA (H3N2) Protein, His Tag (Cat. No. H32-V52H3) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-HA-H3N2(Influenza A/Darwin/6/2021) Antibody, Mouse IgG1 (13A5) (Cat. No. HA2-Y290) with a linear range of 0.1-6 ng/mL (Routinely tested).
Protocol
Immobilized Influenza A Virus (A/Croatia/10136RV/2023) HA (H3N2) Protein, His Tag (Cat. No. H32-V52H4)/Influenza A Virus (A/District of Columbia/27/2023) HA (H3N2) Protein, His Tag (Cat. No. H32-V52H5) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-HA-H3N2(Influenza A/Darwin/6/2021) Antibody, Mouse IgG1 (13A5) (Cat. No. HA2-Y290) with a linear range of 0.2-13 ng/mL (Routinely tested).
Protocol
Bioactivity-SPR

Influenza A [A/Darwin/6/2021 (H3N2)] HA Protein, His Tag (Cat. No. HA2-V52H5) captured on CM5 chip via anti-His antibody can bind Monoclonal Anti-HA-H3N2(Influenza A/Darwin/6/2021) Antibody, Mouse IgG1 (13A5) (Cat. No. HA2-Y290) with an affinity constant of 3.43 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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