PE-Labeled Monoclonal Anti-His Antibody, Mouse IgG1 (55F8)

Customer Reviews
Cat. No. / Size
Price
Qty
HIS-PFY63-25tests
$85.00
HIS-PFY63-200tests
$370.00
ETA of in-stock products:2 business days

Product Details

  • Source

    PE-Labeled Monoclonal Anti-His Antibody, Mouse IgG1 (55F8) is a mouse monoclonal antibody recombinantly expressed from Hybridoma.

  • Antibody Type

    Hybridoma Monoclonal

  • Clone

    55F8

  • Isotype

    Mouse IgG1, Kappa

  • Host Species

    Mouse

  • Reactivity

    His tag (N-terminal, C-terminal and internal His-tagged fusion proteins)

  • Immunogen

    Purified his-tagged Protein

  • Conjugate

    PE

    Excitation Wavelength: 488 nm / 561 nm

    Emission Wavelength: 575 nm

  • Specificity

    Specifically binds to His-tagged proteins without recognizing non-tagged proteins.

  • Application

    Flow Cytometry (Detection the His-tagged protein).

  • Recommended Dilution

    1:50

  • Isotype Control

    The isotype control is sold separately; please refer to Cat. No. DNP-PM1 for product information.

  • Form

    Lyophilized

  • Formulation

    Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.

    Please contact us for customized product forms or formulations.

  • Reconstitution

    Please refer to the Certificate of Analysis (CoA) for specific instructions.

  • Shipping

    Lyophilized product is shipped at ambient temperature.

  • Storage

    For long term storage, the product should be stored in a lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 24 months in lyophilized state;
    2. -70°C for 12 months after reconstitution.
    3. 2-8 °C for 3 months after reconstitution.
  • Notices

    Product Specific Notices: For research use only.

  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

Customers Also Viewed

Performance Data

  • Bioactivity-FACS

     His FACS

    Flow cytometric analysis of Anti-CD19 CAR-293 cells staining with 100 μL of 10 μg/mL Human CD19 (20-291) Protein, His Tag (Cat. No. CD9-H52H2) and negative control protein respectively, washed and then followed with PE-Labeled Monoclonal Anti-His Antibody, Mouse IgG1 (55F8) (Cat. No. HIS-PFY63) at 1:50 dilution (2 μL of the antibody stock solution corresponds to labeling of 1e6 cells in a final volume of 100 µL) and analyzed with FACS. PE signal was used to evaluate the binding activity (QC tested).

    Protocol

Customer Reviews
Writing Reviews

Background

His tag is a very small molecular weight tag, usually composed of 6-10 histidine (His). It is one of the commonly used tags for protein purification and detection. Due to its small molecular weight, fusion into the target protein has almost no effect on the structure and characteristics of the protein. Anti-his tag antibody can accurately detect, locate and purify His tag fusion protein, so as to provide convenience for the vast number of researchers.

Recent Advances

 
Contact Us
Drug Candidate Licensing
More
  • Basic Product Information
  • Product Details
  • Customers Also Viewed
  • Performance Data
  • Customer Reviews
  • Background
Contact Us

[North America]: +1 800-810-0816
[Switzerland]: +41 800 040 012
[Asia & Pacific]: +86 400-682-2521

[North America]: 1 Innovation Way, Newark, DE 19711, USA
[Switzerland]: ACRObiosystems AG, c/o Zug Dammstrasse Centre AG, Dammstrasse 19, 6300 Zug Switzerland