Product Details
Source
Mouse IgG2a Antibody Isotype Control is a monoclonal antibody recombinantly expressed from human 293 cells (HEK293), which provides higher batch consistency and long term security of supply.
Antibody Type
Recombinant Monoclonal
Isotype
Mouse IgG2a, Kappa
Host Species
Mouse
Immunogen
DNP (Dinitrophenyl)
Conjugate
FITC
Excitation source: 488 nm spectral line, argon-ion laser
Excitation Wavelength: 488 nm
Emission Wavelength: 535 nm
Specificity
Specifically reacts with DNP (Dinitrophenyl) and DNP conjugated proteins, which is normally not present in Humans or animals. Therefore, DNP-FM487 does not react with any antigen of Human or animal cells.
Application
Flow Cytometry.
Recommended Dilution
1:50
Form
Lyophilized
Formulation
Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4) containing 0.03% ProClin 300, with trehalose as protectant.
Please contact us for customized product forms or formulations.
Reconstitution
Please refer to the Certificate of Analysis (CoA) for specific instructions.
Shipping
Lyophilized product is shipped at ambient temperature.
Storage
For long term storage, the product should be stored in a lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 24 months in lyophilized state;
- -70°C for 12 months after reconstitution;
- 2-8°C for 12 months after reconstitution.
Notices
Product Specific Notices: For research use only.
ACRO Quality Management System
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Performance Data
Bioactivity-FACS

5e5 of PBMCs were stained with FITC-Labeled Mouse IgG2a Antibody Isotype Control (Cat. No. DNP-FM487), washed and then analyzed with FACS. FITC signal was used to evaluate the non-specific binding activity to PBMCs (QC tested).
Protocol
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