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Human IgG4 (S228P, L235E) Kappa Isotype Control (mAb, carrier free, MALS verified)

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Cat. No. / Size
Price
Qty
DNP-M886-100ug
$165.00
DNP-M886-1mg (500ug X 2)
$720.00
ETA of in-stock products:2 business days
Sub-Total$ 0

Product Details

  • Source

    Human IgG4 (S228P, L235E) Kappa Isotype Control (mAb) is a chimeric monoclonal antibody recombinantly expressed in HEK293, which combines the variable region of a mouse monoclonal antibody with human constant domain.

  • Antibody Type

    Recombinant Monoclonal

  • Isotype

    Human IgG4 (S228P, L235E), Kappa

  • Host Species

    Human

  • Specificity

    This product is a specific antibody specifically reacts with DNP.

  • Cross Verification

    This product No cross-reactivity in ELISA with
    Biotinylated Human CD16b (NA2), His,Avitag (Cat. No. CDB-H82Ea).

  • Purification

    Protein A purified / Protein G purified.

  • Aggregation

    Less than 10%, as determined by SEC-MALS.

  • Concentration

    Please refer to the Certificate of Analysis (CoA).

  • Form

    Lyophilized

  • Formulation

    Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.

    Please contact us for customized product forms or formulations.

  • Reconstitution

    Please refer to the Certificate of Analysis (CoA) for specific instructions.

  • Shipping

    Lyophilized product is shipped at ambient temperature.

  • Storage

    For long term storage, the product should be stored in a lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • Notices

    Product Specific Notices: For research use only.

  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • Bioactivity-ELISA

     IgG control ELISA

    Immobilized Dinitrophenyl-BSA Conjugate (DNP-BSA) at 10 μg/mL (100 μL/well) can bind Human IgG4 (S228P, L235E) Kappa Isotype Control (mAb) (Cat. No. DNP-M886) with a linear range of 0.02-2 ng/mL (QC tested).

    Protocol
  •  IgG control ELISA

    Immobilized Human IgG4 (S228P, L235E) Kappa Isotype Control (mAb) (Cat. No. DNP-M886) at 1 μg/mL (100 μL/well) can bind Biotinylated Human CD16a (V176) Protein, Avitag,His Tag (Cat. No. CDA-H82E9) with a linear range of 0.016-1 μg/mL (Routinely tested).

    Protocol

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Background

Fc engineered isotype controls are designed to evaluate effector functions such as FcγR binding, FcRn interaction, ADCC, and CDC. Each variant is functionally validated, providing a reliable negative control framework for antibody mechanism studies and therapeutic development.

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