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Product Details
Background
Recombinant Monoclonal Anti-Ki-67 Antibody, Rabbit (7A4) is a Ki-67 primary antibody for IHC application. Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly . Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface. Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility.
Product Specifications
Host SpeciesRabbitIsotypeRabbit IgG, Rabbit KappaClone7A4ApplicationIHC-PDilution Ratio1: 200 - 1: 1000FormLiquidClonalityMonoclonalReactivityHumanSynonymsPPP1R105, MIB-1, ki-67Concentration0.9~1.1 mg/mlSpecificityReact with Ki-67 protein on the intracellular area of target cellsPurification TechProtein APreservationTris buffer, 1% BSAExperiment Protocol
Storage
Shipped at 4°C, Blue Ice. Store at +4°C for short term (1-2 weeks). Store at -20°C for long term.
Avoid freeze / thaw cycle. Upon delivery aliquot. The shelf life is 30 days from the date of opening.ACRO Quality Management System
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Performance Data
Sample Staining Data
Immunohistochemical analysis of paraffin embedded human cancer tissue labelled with HGS-S239 at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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