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Product Details
Product Overview
Human Serum Albumin ELISA Kit is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant human serum albumin that is present in biological samples, such as serum, and cell culture supernatants. Our ELISA Kit provides several benefits:
- Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
- High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
- Simplified and straightforward protocols and ready-to-use reagents to save assay time.
Product Specifications
Assay TypeSandwich-ELISAAnalyteSerum AlbuminFormat96-wells plate breakable into 12 x 8 wells stripsReactivityHumanSensitivity2.5 ng/mLAssay Time2.75 hrSample volume50 μLRange3.125 ng/mL-200 ng/mLSample TypeFor the quantitative determination of Human Serum Albumin in serum, plasma and cell supernatant.Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.
Application
The kit is developed for quantitative detection of natural and recombinant human serum albumin in serum, and cell culture supernatants.
It is for research use only.
Workflow
Storage
Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Materials Provided
IDComponentsSizeCEA260-C01Pre-coated Anti-HSA Antibody Microplate1 plateCEA260-C02HSA Standard5 μg/mL×2CEA260-C03Biotin-Anti-HSA Antibody Con. Solution100 μLCEA260-C04Biotin-Antibody Dilution Buffer8 mLCEA260-C05Streptavidin-HRP Con. Solution500 μLCEA260-C06Streptavidin-HRP Dilution Buffer15 mLCEA260-C0720× Washing Buffer50 mLCEA260-C08Sample Dilution Buffer15 mL×2CEA260-C09Substrate Solution12 mLCEA260-C10Stop Solution6 mLACRO Quality Management System
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Performance Data
Typical Data
Please refer to DS document for the assay protocol.
For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of HSA is less than 2.5 ng/mL.
Intra-Assay Statistics
Ten replicates of each of 3 samples containing different HSA concentrations were tested in one assay. Acceptable criteria: CV＜10%.
Inter-Assay Statistics
Three samples containing different concentrations of HSA were tested in independent assays. Acceptable criteria: CV<15%.
Recovery
Recombinant HSA was spiked into 3 human samples, and then analyzed. The average recovery of HSA for samples is 102.4%.
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Drug Development Progress- English Name：
Serum albumin
- Category：
- Approved Drugs：
17 Details
- Drugs in Clinical Trials：
12 Details
- Highest Development Stage：
Approved
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