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Product Details
Product Overview
Mouse TL1A/TNFSF15 ELISA Kit is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant mouse TL1A/TNFSF15 that is present in biological samples, such as mouse serum, plasma, and cell culture supernatants. Our ELISA Kit provides several benefits:
- Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
- High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
- Simplified and straightforward protocols and ready-to-use reagents to save assay time.
Product Specifications
Assay TypeSandwich-ELISAAnalyteTL1AFormat96-wells plate breakable into 12 x 8 wells stripsReactivityMouseSensitivity5 pg/mLAssay Time2 hr 45 minSample volume50 μLRange9.375 pg/mL-600 pg/mLSample TypeFor the quantitative determination of mouse TL1A in serum, plasma and cell supernatant.Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.
Application
The kit is developed for quantitative detection of natural and recombinant mouse TL1A in serum, plasma and cell culture supernatants.
It is for research use only.
Workflow
Storage
Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Materials Provided
IDComponentsSizeCEA259-C01Pre-coated Anti-TL1A Antibody Microplate1 plateCEA259-C02Mouse TL1A Standard12 μg×2CEA259-C03Biotin-Anti-TL1A Antibody Con. Solution100 μLCEA259-C04Biotin-Antibody Dilution Buffer8 mLCEA259-C05Streptavidin-HRP Con. Solution500 μLCEA259-C06Streptavidin-HRP Dilution Buffer15 mLCEA259-C0720×Washing Buffer50 mLCEA259-C08Sample Dilution Buffer15 mL×2CEA259-C09Substrate Solution12 mLCEA259-C10Stop Solution6 mLACRO Quality Management System
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Performance Data
Typical Data
Please refer to DS document for the assay protocol.
For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of TL1A is less than 5 pg/mL.
Intra-Assay Statistics
Ten replicates of each of three samples containing different mouse TL1A concentrations were tested in one assay. Acceptable criteria: CV＜10%.
Inter-Assay Statistics
Three samples containing different concentrations of mouse TL1A were tested in independent assays. Acceptable criteria: CV<15%.
Recovery
Recombinant TL1A was spiked into three mouse serum samples, and then analyzed. The average recovery of TL1A for serum samples is 107.67%.
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