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Cynomolgus ASGR1 Protein, Fc Tag, low endotoxin

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Cat. No. / Size
Price
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AS1-C5253-100ug
$395.00
AS1-C5253-1mg
$2500.00
ETA of in-stock products:2 business days
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Product Details

  • Synonyms

    ASGP-R 1, ASGPR 1, C-type lectin domain family 4 member H1, Hepatic lectin H1, HL-1

  • Source

    Cynomolgus ASGR1 Protein, Fc Tag (AS1-C5253) is expressed from human 293 cells (HEK293). It contains AA Ser 61 - Leu 291 (Accession # XP_005582755.1).

    Predicted N-terminus: Pro

    Request for sequence
  • Molecular Characterization

    ASGR1 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a human IgG1 Fc tag at the N-terminus.

    The protein has a calculated MW of 52.9 kDa. The protein migrates as 55 kDa and 60-65 when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 0.01 EU per μg by the LAL method / rFC method.

  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    ASGR1 SDS-PAGE

    Cynomolgus ASGR1 Protein, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • Bioactivity-ELISA

     ASGR1 ELISA

    Immobilized Cynomolgus ASGR1 Protein, Fc Tag (Cat. No. AS1-C5253) at 1 μg/mL (100 μL/well) can bind Mouse Anti-Human ASGR1 antibody-4G5, Mouse IgG2b|Mouse Kappa with a linear range of 0.06-1 ng/mL (QC tested).

    Protocol

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Background

This gene encodes a subunit of the asialoglycoprotein receptor. This receptor is a transmembrane protein that plays a critical role in serum glycoprotein homeostasis by mediating the endocytosis and lysosomal degradation of glycoproteins with exposed terminal galactose or N-acetylgalactosamine residues. The asialoglycoprotein receptor may facilitate hepatic infection by multiple viruses including hepatitis B, and is also a target for liver-specific drug delivery. The asialoglycoprotein receptor is a hetero-oligomeric protein composed of major and minor subunits, which are encoded by different genes. The protein encoded by this gene is the more abundant major subunit. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2011]


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Drug Development Progress
  • English Name:

    Asialoglycoprotein receptor 1

  • Category:

  • Approved Drugs:

    0 Details

  • Drugs in Clinical Trials:

    1 Details

  • Highest Development Stage:

    Phase 2 Clinical

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