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Human BTN2A1 / Butyrophilin Protein, His Tag, low endotoxin (MALS verified)

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Cat. No. / Size
Price
Qty
BT1-H52H6-100ug
$395.00
BT1-H52H6-1mg (500ug X 2)
$2615.00
ETA of in-stock products:2 business days
Sub-Total$ 0

Product Details

  • Synonyms

    BK14H9.1, BT2.1, BTF1, BTF1BT2.1butyrophilin BTF1, BTN2A1, butyrophilin subfamily 2 member A1, butyrophilin, subfamily 2, member A1, DJ3E1.1, FLJ36567

  • Source

    Human BTN2A1 Protein, His Tag (BT1-H52H6) is expressed from human 293 cells (HEK293). It contains AA Gln 29 - Ala 248 (Accession # Q7KYR7-2).

    Predicted N-terminus: Gln 29

    Request for sequence
  • Molecular Characterization

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 26.4 kDa. The protein migrates as 38-45 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 0.01 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    BTN2A1 SDS-PAGE

    Human BTN2A1 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    BTN2A1 SEC-MALS

    The purity of Human BTN2A1 Protein, His Tag (Cat. No. BT1-H52H6) is more than 95% and the molecular weight of this protein is around 30-46 kDa verified by SEC-MALS.

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Background

Duplication events have led to three paralogues of BTN2A in primates: BTN2A1, BTN2A2, and BTN2A3. In humans, only BTN2A1 has been functionally characterised; it has been detected on epithelial cells and leukocytes, and identified as a novel ligand of dendritic cell-specific ICAM-3 grabbing nonintegrin (DCSIGN), a C-type lectin receptor that acts as an internalization receptor for HIV-1, HCV, and other pathogens. BTN2A2 mRNA has been shown to be expressed in circulating human immune cells.

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