Product Details
Synonyms
CD19, B4, CVID3, MGC12802
Source
Biotinylated Mouse CD19 Protein, His Tag, primary amine labeling (CD9-M82H6) is expressed from human 293 cells (HEK293). It contains AA Arg 19 - Gly 287 (Accession # P25918). It is the biotinylated form of Mouse CD19, His Tag (Cat. No. CD9-M52H8).
Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 31.6 kDa. The protein migrates as 43-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE

Biotinylated Mouse CD19 Protein, His Tag, primary amine labeling on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
Bioactivity-ELISA

Immobilized CD19 Monoclonal Antibody at 1 μg/mL (100 μL/well) can bind Biotinylated Mouse CD19 Protein, His Tag, primary amine labeling (Cat. No. CD9-M82H6) with a linear range of 0.005-0.156 μg/mL (QC tested).
Protocol
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Background
Recent Advances
- English Name:
B-lymphocyte antigen CD19
- Category:
- Approved Drugs:
14 Details
- Drugs in Clinical Trials:
435 Details
- Highest Development Stage:
Approved
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