Product Details
Synonyms
CD27, TNFRSF7, S152, T14, Tp55
Source
Cynomolgus / Rhesus macaque CD27 Protein, Mouse IgG2a Fc Tag (CD7-C5259) is expressed from human 293 cells (HEK293). It contains AA Ala 20 - Arg 191 (Accession # XP_005569963.1). In the region Ala 20 - Arg 191, the amino acid sequences of Cynomolgus and Rhesus macaque CD27 are identical.
Predicted N-terminus: Ala 20
Request for sequenceMolecular Characterization

Other Tags and Version Biotin & Other Labeled Version
This protein carries a mouse IgG2a Fc tag at the C-terminus.
The protein has a calculated MW of 46.2 kDa. The protein migrates as 50-66 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE

Cynomolgus / Rhesus macaque CD27 Protein, Mouse IgG2a Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS

The purity of Cynomolgus / Rhesus macaque CD27 Protein, Mouse IgG2a Fc Tag (Cat. No. CD7-C5259) is more than 95% and the molecular weight of this protein is around 105-135 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA

Immobilized Cynomolgus / Rhesus macaque CD27 Protein, Mouse IgG2a Fc Tag (Cat. No. CD7-C5259) at 2 μg/mL (100 μL/well) can bind Biotinylated Human CD27 Ligand, Fc Tag with a linear range of 2-31 ng/mL (QC tested).
Protocol
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Background
Recent Advances
- English Name:
CD27 antigen
- Category:
- Approved Drugs:
0 Details
- Drugs in Clinical Trials:
4 Details
- Highest Development Stage:
Phase 2 Clinical
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