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Canine CD3 epsilon Protein, His Tag, low endotoxin (MALS verified)

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Cat. No. / Size
Price
Qty
CDE-C52H3-100ug
$395.00
CDE-C52H3-1mg (500ug X 2)
$2470.00
ETA of in-stock products:2 business days
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Product Details

  • Synonyms

    FLJ18683, T3E, TCRE, CD3E, CD3-epsilon

  • Source

    Canine CD3 epsilon Protein, His Tag (CDE-C52H3) is expressed from human 293 cells (HEK293). It contains AA Gln 22 - Leu 122 (Accession # P27597).

    Predicted N-terminus: Gln 22

  • Molecular Characterization

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 16.7 kDa. The protein migrates as 19-25 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

    The protein is designed as a dimer.

  • Endotoxin

    Less than 0.01 EU per μg by the LAL method / rFC method.

  • Purity

    >90% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    CD3 epsilon SDS-PAGE

    Canine CD3 epsilon Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    CD3 epsilon SEC-MALS

    The purity of Canine CD3 epsilon Protein, His Tag (Cat. No. CDE-C52H3) is more than 95% and the molecular weight of this protein is around 32-45 kDa verified by SEC-MALS.

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Background

CD3e molecule, epsilon is also known as CD3E, is a T-cell surface single-pass type I membrane glycoprotein. CD3E contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3E, together with CD3-gamma, CD3-delta and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. CD3E plays an essential role in T-cell development, and defects in CD3E gene cause severe immunodeficiency. CD3E gene has also been linked to a susceptibility to type I diabetes in women. CD3E has been shown to interact with TOP2B, CD3EAP and NCK2.

Recent Advances

 
Drug Development Progress
  • English Name:

    T-cell surface glycoprotein CD3 epsilon chain

  • Category:

  • Approved Drugs:

    4 Details

  • Drugs in Clinical Trials:

    7 Details

  • Highest Development Stage:

    Approved

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