PE-Labeled Human CD30 / TNFRSF8 Protein, His Tag (Site-specific conjugation)

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Cat. No. / Size
Price
Qty
CD0-HP2E3-25tests
$360.00
CD0-HP2E3-200tests
$2245.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    TNFRSF8, CD30, D1S166E, Ki-1

  • Source

    PE-Labeled Human CD30, His Tag (CD0-HP2E3) is produced via site-specific conjugation of PE to Human CD30, His Tag under optimal conditions with a proprietary technology. Human CD30, His Tag is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Lys 379 (Accession # NP_001234.2).

    Predicted N-terminus: Phe 19

    Request for sequence

  • Molecular Characterization

    CD30 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 41.1 kDa.

  • Conjugate

    PE

    Excitation Wavelength: 488 nm / 561 nm

    Emission Wavelength: 575 nm

  • Application

    Evaluation of anti-CD30 CAR expression by flow cytometry. Please note that this product is NOT compatible to streptavidin detection system.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, 0.5% BSA, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • Bioactivity-FACS

     CD30 FACS

    5e5 of anti-CD30 CAR-293 cells were stained with 100 μL of 1:50 dilution (2 μL stock solution in 100 μL FACS buffer) of PE-Labeled Human CD30, His Tag (Cat. No. CD0-HP2E3) and negative control protein respectively. PE signal was used to evaluate the binding activity (QC tested).

    Protocol

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Background

Human CD30 is also known as TNFRSF8, is a cell membrane protein of the tumor necrosis factor receptor family and tumor marker. TNFRSF-8 is expressed by activated, but not by resting, T and B cells. Also, CD30 is expressed on classical Hodgkin Lymphoma cells together with CD15. CD30 is the receptor for TNFSF8/CD30L. CD30 can interact with TRAF2 and TRAF5, and mediate the signal transduction that leads to the activation of NF-kappa-B. TNFRSF8 may play a role in the regulation of cellular growth and transformation of activated lymphoblasts. TNFRSF8 is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity.

Recent Advances

 
Drug Development Progress
  • English Name:

    Tumor necrosis factor receptor superfamily member 8

  • Category:

  • Approved Drugs:

    1 Details

  • Drugs in Clinical Trials:

    22 Details

  • Highest Development Stage:

    Approved

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