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Product Details
Application
Synonyms
Claudin-18.2, CLDN18, Claudin-18
Source
Nanodisc Human / Cynomolgus Claudin-18.2, His,Twin-Strep Tag (CL2-H5586) is expressed from Baculovirus-Insect cells. It contains AA Met 1 - Ala 200 (Accession # P56856-2). In the region Met 1 - Ala 200, the amino acid sequences of Human and Cynomolgus Claudin-18.2 are identical.
Predicted N-terminus: Met
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the N-terminus and a twin strep tag at the C-terminus.
The protein has a calculated MW of 26.5 kDa.
*The isotype control of empty/mock nanodisc (Cat. No. APO-H51H3) is sold separately and not included in protein, you can follow the link for product information.
Structure
Nanodisc membrane protein is wrapped by a synthetic phospholipid bilayer membrane structure, composed of membrane scaffold proteins (MSPs) and phospholipids.
Synthesis Process
Nanodisc reconstitution is a self-assembly process that starts from purified, detergent-solubilized membrane proteins and reconstitutes them into synthetic phospholipid bilayers stabilized by MSP1D1 membrane scaffold protein. This creates a stable, detergent-free environment that preserves native conformation and biological activity, with excellent homogeneity and full compatibility with cell-based assays such as FACS and binding assays in antibody discovery and screening (ELISA),etc.
Formulation
Supplied as 0.2 μm filtered solution in 20 mM HEPES, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Shipping and Storage
This product is supplied and shipped on dry ice.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
ACRO Quality Management System
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Performance Data
Bioactivity-ELISA
Immobilized Human / Cynomolgus Claudin-18.2, His,Twin-Strep Tag (Cat. No. CL2-H5586) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-Chimeric Claudin-18.2 Antibody, Human IgG1 with a linear range of 0.1-2 ng/mL (QC tested).
Protocol
Bioactivity-SPR
Monoclonal Anti-Chimeric Claudin-18.2 Antibody, Human IgG1 captured on Protein A Chip can bind Human / Cynomolgus Claudin-18.2, His,Twin-Strep Tag (Cat. No. CL2-H5586) with an affinity constant of 12.6 nM as determined in SPR assay (Biacore 8K) (Routinely tested).
Protocol
Bioactivity-FACS
2e5 of Anti-Claudin18.2 CAR-293 cells were stained with 100 μL of 3 μg/mL Human Claudin-18.2 Protein, His,Twin-Strep Tag (Nanodisc)(Cat. No. CL2-H5586) and isotype control protein respectively, washed and then followed by PE anti-His Tag Antibody and analyzed with FACS (QC tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Claudin-18 splice variant 2
- Category：
- Approved Drugs：
1 Details
- Drugs in Clinical Trials：
82 Details
- Highest Development Stage：
Approved
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