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Product Details
Synonyms
CLEC5A, CLECSF5, superfamilymember 5, C-type lectin domain family 5, member A, MDL1, MDL-1, DAP12-associating lectin 1
Source
Human CLEC5A Protein, Fc Tag (CLA-H5263) is expressed from human 293 cells (HEK293). It contains AA Pro 28 - Lys 188 (Accession # Q9NY25-1).
Predicted N-terminus: Pro
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the N-terminus.
The protein has a calculated MW of 44.8 kDa. The protein migrates as 53-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human CLEC5A Protein, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human CLEC5A Protein, Fc Tag (Cat. No. CLA-H5263) is more than 95% and the molecular weight of this protein is around 90-120 kDa verified by SEC-MALS.
Report
Bioactivity-SPR
Recombinant Human Galectin-9 Protein, CF (E.Coli) immobilized on CM5 Chip can bind Human CLEC5A Protein, Fc Tag (Cat. No. CLA-H5263) with an affinity constant of 0.0145 nM as determined in a SPR assay (Biacore 8K) (QC tested).
Protocol
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