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Product Details
Synonyms
TNFRSF25, DR3, APO3, DDR3, TNFRSF12, WSL, WSL1
Source
Human DR3 Protein, Fc Tag (DR3-H5253) is expressed from human 293 cells (HEK293). It contains AA Gln 25 - Gln 199 (Accession # Q93038-1).
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 45.5 kDa. The protein migrates as 53-63 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human DR3 Protein, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human DR3 Protein, Fc Tag (Cat. No. DR3-H5253) is more than 95% and the molecular weight of this protein is around 95-115 kDa verified by SEC-MALS.
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Bioactivity-ELISA
Immobilized Human TL1A, His Tag (Cat. No. TLA-H5243) at 10 μg/mL (100 μL/well) can bind Human DR3 Protein, Fc Tag (Cat. No. DR3-H5253) with a linear range of 1-78 ng/mL (QC tested).
Protocol
Immobilized Human DR3, Fc Tag (Cat. No. DR3-H5253) at 5 μg/mL (100 μL/well) can bind pre-mixed increasing concentrations of Anti-TL1A Neutralizing Antibody, Human lgG1 and 1 μg/mL (50μL/well) Biotinylated Human TL1A, His Avitag (Cat.No.TLA-H52Q1) with a half maximal inhibitory concentration (lC50) of 1.95 μg/mL (Routinely tested).
Protocol
Bioactivity-SPR
Human TL1A, His Tag (Cat. No. TLA-H5243) captured on CM5 chip via anti-His antibody can bind Human DR3 Protein, Fc Tag (Cat. No. DR3-H5253) with an affinity constant of 1.15 µM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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