PE-Labeled Human EGF R Protein, His Tag (Site-specific conjugation)

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Cat. No. / Size
Price
Qty
EGR-HP2E3-25tests
$415.00
EGR-HP2E3-200tests
$2570.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    EGFR, ERBB, ERBB1, HER1, PIG61, mENA

  • Source

    PE-Labeled Human EGF R, His Tag (EGR-HP2E3) is produced via site-specific conjugation of PE to Human EGF R, His Tag under optimal conditions with a proprietary technology. Human EGF R, His Tag is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Ser 645 (Accession # P00533-1).

    Predicted N-terminus: Leu 25

    Request for sequence

  • Molecular Characterization

    EGF R Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 72.3 kDa.

  • Conjugate

    PE

    Excitation Wavelength: 488 nm / 561 nm

    Emission Wavelength: 575 nm

  • Application

    Evaluation of anti-EGF R CAR expression by flow cytometry. Please note that this product is NOT compatible to streptavidin detection system.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, 0.5% BSA, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • Bioactivity-FACS

     EGF R FACS

    5e5 of anti-EGFR CAR-293 cells were stained with 100 μL of 1:50 dilution (2 μL stock solution in 100 μL FACS buffer) of PE-Labeled Human EGF R, His Tag (Cat. No. EGR-HP2E3) and negative control protein respectively. PE signal was used to evaluate the binding activity (QC tested).

    Protocol

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Background

The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). Mutations affecting EGFR expression or activity could result in cancer.

Recent Advances

 
Drug Development Progress
  • English Name:

    Epidermal growth factor receptor erbB1

  • Category:

  • Approved Drugs:

    39 Details

  • Drugs in Clinical Trials:

    206 Details

  • Highest Development Stage:

    Approved

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